Effects of HER2 shRNA on Chemotherapy Sensitivity of Mouse Lewis Cells
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摘要: 目的 探讨靶向HER2基因shRNA对小鼠肺腺癌Lewis细胞化疗敏感度的影响。方法 应用Lipofectamine 2000将pGPU6/RFP/Neo-erbB-2、pGPU6/RFP/Neo-shNC质粒表达载体快速转染小鼠肺腺癌Lewis细胞；应用RT-PCR、Western blot检测各组HER2 mRNA、蛋白的表达。应用流式细胞术检测未转染对照组、pGPU6/RFP/Neo-shNC组、pGPU6/RFP/Neo-erbB-2组、卡铂组、pGPU6/RFP/Neo-shNC+卡铂组、pGPU6/RFP/Neo-erbB-2+卡铂组的细胞凋亡率。结果 pGPU6/RFP/Neo-erbB-2组HER2 mRNA 和蛋白的水平均低于pGPU6/RFP/Neo-shNC组和未转染对照组。pGPU6/RFP/Neo-erbB-2+卡铂组细胞的凋亡率高于其余各组（P均＜0.01）。结论 HER2 shRNA能增强小鼠Lewis细胞对卡铂的敏感度。Abstract: Objective To investigate the effects of HER2 shRNA on the chemotherapy sensitivity of Mice Lewis cells. Methods pGPU6/RFP/Neo-erbB-2 and pGPU6/RFP/Neo-shNC plasmids were synthesized and transfected into mice Lewis cells by Lipofectamine 2000.The levels of HER2 mRNA and protein were tested by RT-PCR and Western blot, respectively.The apoptotic rates of cells in non-transfected group, pGPU6/RFP/Neo-shNC group,pGPU6/RFP/Neo-erbB-2 group,Carboplatin group, pGPU6/RFP/Neo-shNC plus Carboplatin group and pGPU6/RFP/Neo-erbB-2 plus Carboplatin group were tested by FCM. Results The levels of HER2 mRNA and protein in pGPU6/RFP/Neo-erbB-2 group were lower than those in pGPU6/RFP/Neo-shNC group and non-transfected group.The apoptotic rate of cells in pGPU6/RFP/Neo-erbB-2 plus carboplatin group was higher than those in other groups (all P＜0.01). Conclusion HER2 shRNA could increase the sensitivity of mouse Lewis cells to carboplatin.
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Key words:
- HER2 /
- shRNA /
- Lewis cells /
- Chemotherapy sensitivity
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