Mechanism of TNFα Regulating KLF4 Expression in Breast Cancer Cells SK-BR-3

LIU Caiyun; SHI Jianhong; ZHOU Jinsa; ZHAO Mingzhi; WANG Bing; CUI Naipeng; CHEN Baoping

doi:10.3971/j.issn.1000-8578.2015.02.004

Cancer Research on Prevention and Treatment > 2015 > 42(02): 117-120. > DOI: 10.3971/j.issn.1000-8578.2015.02.004

LIU Caiyun, SHI Jianhong, ZHOU Jinsa, ZHAO Mingzhi, WANG Bing, CUI Naipeng, CHEN Baoping. Mechanism of TNFα Regulating KLF4 Expression in Breast Cancer Cells SK-BR-3[J]. Cancer Research on Prevention and Treatment, 2015, 42(02): 117-120. DOI: 10.3971/j.issn.1000-8578.2015.02.004

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Mechanism of TNFα Regulating KLF4 Expression in Breast Cancer Cells SK-BR-3

1. Department of Oncology, Affiliated Hospital of Hebei University, Baoding 071000, China;2. Central Laboratory, Affiliated Hospital of Hebei University, Baoding 071000, China; 3.Clinical Medical College of Hebei University, Baoding 071000, China

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Received Date: January 29, 2014
Revised Date: April 01, 2014

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Abstract

Objective To investigate the expression level and role of Krüpple-like factor 4 (KLF4) in tumor necrosis factor α (TNFα) stimulated breast cancer cells SK-BR-3, and to identify the related mechanism. Methods Breast cancer cells SK-BR-3 were stimulated by TNFα at different concentrations (0, 1, 5, 10, 20 ng/mL) for 24 h. Adenovirus expression vectors of pAd-GFP and pAd-GFP-KLF4 were constructed and used to infect breast cancer cells SK-BR-3.Western blot was performed to detect KLF4 expression level. Flow cytometry and DAPI staining were used to investigate cell apoptosis. Results KLF4 expression levels were increased significantly in TNFα-stimulated breast cancer cells SK-BR-3 with more TNFα concentration. Flow cytometry and DAPI staining results showed that TNFα induced SK-BR-3 apoptosis. Flow cytometry results showed KLF4 overexpression promoted the apoptosis of TNFα-stimulated breast cancer cells SK-BR-3. Conclusion TNFα could induce KLF4 expression in breast cancer cells SK-BR-3, and KLF4 participates in cell apoptosis of TNFα-induced breast cancer cells SK-BR-3.
- Tumor necrosis factor α Breast cancer cells SK-BR-3,
- Krüpple-like factor 4,
- Apoptosis

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Mechanism of TNFα Regulating KLF4 Expression in Breast Cancer Cells SK-BR-3

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