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ZHANG Qiao, XING Rui-ting, XU Hong-hui, YUAN Jin-song, LI Chun-yang, WU Wei- dong, WU Yi-ming, CHENG Shu-jun. Construction of Eukaryotic Expression Vector of Rap2b and Its Effects on Pathway of NIH3T3 Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(06): 640-643. DOI: 10.3971/j.issn.1000-8578.2010.06.009
Citation: ZHANG Qiao, XING Rui-ting, XU Hong-hui, YUAN Jin-song, LI Chun-yang, WU Wei- dong, WU Yi-ming, CHENG Shu-jun. Construction of Eukaryotic Expression Vector of Rap2b and Its Effects on Pathway of NIH3T3 Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(06): 640-643. DOI: 10.3971/j.issn.1000-8578.2010.06.009
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Construction of Eukaryotic Expression Vector of Rap2b and Its Effects on Pathway of NIH3T3 Cells

  • 1.Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China; 2.Cancer Institute of Chinese Academy of Medical Sciences, Beijing 100021,China；3.Zhanjiang Institute for Prevention and Treatment of Occupational Disease

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  • Received Date: April 06, 2009
  • Revised Date: October 19, 2009
    Abstract
  • Objective To construct pcDNA3.1-Rap2b eukaryotic expression vector and to understand the effects on AKT, ERK, JNK and P38 signaling pathway of NIH3T3 cells through transfecting Rap2b gene into NIH3T3 cells. Methods Eukaryotic expression vector pcDNA3.1-Rap2b was constructed and was stable transfected into NIH3T3 cell, and its effects on the AKT、ERK、JNK and P38 pathway of NIH3T3 cells were observed by Western blotting to measure the level of the expression of AKT、ERK、JNK and P38 Phosphorylation protein and total protein. Results The level of the expression of P38 Phosphorylation protein was higher in the pcDNA3.1-Rap2b vector than that of control（P＜0.05）, and the level of the expression of AKT、ERK、JNK Phosphorylation protein was not different comparing with the control（P＞0.05）. Conclusion Rap2b might activate P38 signaling pathway, but not activate AKT, ERK, JNK signaling pathway.
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    CHENG Shu-jun

    • 1.Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China; 2.Cancer Institute of Chinese Academy of Medical Sciences, Beijing 100021,China；3.Zhanjiang Institute for Prevention and Treatment of Occupational Disease

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