Stat3 Signaling Pathway Regulates the Expression of Caspase-3 and Promotes Apoptosis of Human Colon Cancer Cells

Objective The purpose of the study was to examine colon cancer cell lines to determine whether Stat3/Caspase-3 plays an important role in the process of apoptosis in colon cancer cells. Methods Protein lysates were extracted from colon cancer cells. Human colon cancer cell line HT29 was transfected with Stat3 antisense oligonucleotide mediated by liposome, MTT assay was used to measure the proliferation, flow cytometry was applied to analyze the cell cycle and apoptosis, the expression of Stat3, p-Stat3, Ca...