Establishment of Human Colorectal Cancer Lymphatic Metastasis Model in Nude Mice

bjective To establish the simple,viable and stably nude mice model of lymphatic metastasis using human colorectum tumor. Methods The lymphatic metastasis nude models were established by injecting colorectum tumor HCT-116 cell line into the nail pad.Detect the formation rate,growing condition and metastasis regularity,collect the drain part at different moments on weeks 1～6,and 8 after tumor implantation in different bathes,including groin popliteal fossa,ilio-blood-vessel side,renal hilum lymph node and the distant organ.Then detect the HE dyeing and immunohistochemistry stain of CEA. Results The formation rate is 100% after 1 week.Generate metastasis 3 weeks later.Until inject tumor cells 6 weeks later,groin popliteal fossa lymph nodes metastasis rate is 100%,including 76.9％（10/13）of groin lymphatic metastasis; ilio-blood-vessel side metastasis rate is 40%(2/5);no finding in renal hilum lymph node metastasis.In 8th week ilio-blood-vessel side lymph nodes metastasis rate is 60%(3/5),renal hilar lymph node generate metastasis,but no finding metastasis in liver,lungs.Detected diffuse cancer cell metastasis in lymph nodes using HE dyeing and immunohistochemistry stain of CEA. Conclusion The human colorectal cancer simple lymphatic metastasis model can be successfully established through the Claw pad hypodermic in nude mice.The model is simple,rapid,concentrated transfer and high rate transfer.It is an ideal animal model for colorectal cancer study lymphatic metastasis mechanism and anti-drug intervention transfer therapy.