2006 Vol. 33 No. 08
2006, 33(08): 551-554.
DOI: 10.3971/j.issn.1000-8578.1848
Abstract：
Objective Squamous dysplasia is the precursor lesion for esophageal squamous cell carcinoma (ESCC). A primary screening test for ESCC which identified this lesion could lead to a reduction in disease-specific mortality and provide clues to early detection of ESCC. Methods We conducted a population-based screening study in Linzhou, China, a region with high rates of ESCC. All subjects provided blood samples and underwent endoscopy with Lugol s iodine staining and biopsy. We selected a subset of 84 subjects st...
Objective Squamous dysplasia is the precursor lesion for esophageal squamous cell carcinoma (ESCC). A primary screening test for ESCC which identified this lesion could lead to a reduction in disease-specific mortality and provide clues to early detection of ESCC. Methods We conducted a population-based screening study in Linzhou, China, a region with high rates of ESCC. All subjects provided blood samples and underwent endoscopy with Lugol s iodine staining and biopsy. We selected a subset of 84 subjects st...
2006, 33(08): 555-557.
DOI: 10.3971/j.issn.1000-8578.1846
Abstract：
Objective To investigate the expression and clinicopathological significance of PINCH protein in esophagus squamous cell carcinoma. Methods ABC immunohistochemical method was used to detect the expression of PINCH in paraffin block from 10 cases normal esophagus mucous tissue and 64 cases esophagus squamous cell carcinoma, and to study the significance of expression of PINCH protein. Results PINCH protein expression in tumor-associated stromal cells of esophagus squamous cell carcinoma was heterogeneous and i...
Objective To investigate the expression and clinicopathological significance of PINCH protein in esophagus squamous cell carcinoma. Methods ABC immunohistochemical method was used to detect the expression of PINCH in paraffin block from 10 cases normal esophagus mucous tissue and 64 cases esophagus squamous cell carcinoma, and to study the significance of expression of PINCH protein. Results PINCH protein expression in tumor-associated stromal cells of esophagus squamous cell carcinoma was heterogeneous and i...
2006, 33(08): 558-560.
DOI: 10.3971/j.issn.1000-8578.1832
Abstract：
Objective Our purpose was to explore the clinical significance of MGMT and GST-π protein expression in breast carcinoma tissues and corresponding para-carcinoma tissues. Methods Flow cytometry was used to analyze the expression of MGMT and GST-π protein. Results The expression of MGMT and GST-π were higher in breast carcinoma tissues than that in corresponding para-carcinoma tissues(P< 0.05).There were not notable differences of two drug resistance related protein expressions in different ages,different tu...
Objective Our purpose was to explore the clinical significance of MGMT and GST-π protein expression in breast carcinoma tissues and corresponding para-carcinoma tissues. Methods Flow cytometry was used to analyze the expression of MGMT and GST-π protein. Results The expression of MGMT and GST-π were higher in breast carcinoma tissues than that in corresponding para-carcinoma tissues(P< 0.05).There were not notable differences of two drug resistance related protein expressions in different ages,different tu...
2006, 33(08): 561-564.
DOI: 10.3971/j.issn.1000-8578.1831
Abstract：
Objective To investigate the law during cisplatin-induced apoptosis in cisplatin-resistant and sensitive human ovarian cancer cell line A2780 and A2780/DDP and correlative protein expressions in mitochondria apoptotic pathway. Methods Two ovarian cancer cell lines,one is cisplatin-sensitive line A2780 and the other is resistant-line A2780/DDP,were selected.Based on cell culture,the influence of varied doses(5,10,20,40μM) and time(24,48,72h) of cisplatin on apoptosis in A2780 and A2780/DDP cell lines and prot...
Objective To investigate the law during cisplatin-induced apoptosis in cisplatin-resistant and sensitive human ovarian cancer cell line A2780 and A2780/DDP and correlative protein expressions in mitochondria apoptotic pathway. Methods Two ovarian cancer cell lines,one is cisplatin-sensitive line A2780 and the other is resistant-line A2780/DDP,were selected.Based on cell culture,the influence of varied doses(5,10,20,40μM) and time(24,48,72h) of cisplatin on apoptosis in A2780 and A2780/DDP cell lines and prot...
2006, 33(08): 565-567.
DOI: 10.3971/j.issn.1000-8578.1830
Abstract：
Objective To investigate the difference of DNA copy number and gene expression on chromosome and their correlation with taxol-resistance of ovarian carcinoma. Methods Comparative genomic hybridization and RT-PCR technique were performed on the taxol-resistant cell lines and ovarian cancer tissues to compare the genomic alteration of chromosome and the hmsh2 gene expression. Results CGH results showed that the most interesting finding was the amplification of 2p22 in OC3/Tax300.Over-expression of hmsh2 gene in...
Objective To investigate the difference of DNA copy number and gene expression on chromosome and their correlation with taxol-resistance of ovarian carcinoma. Methods Comparative genomic hybridization and RT-PCR technique were performed on the taxol-resistant cell lines and ovarian cancer tissues to compare the genomic alteration of chromosome and the hmsh2 gene expression. Results CGH results showed that the most interesting finding was the amplification of 2p22 in OC3/Tax300.Over-expression of hmsh2 gene in...
2006, 33(08): 568-571.
DOI: 10.3971/j.issn.1000-8578.1829
Abstract：
Objective To examine the potential of HPV16 E7 and Brn-3a as a biomarker for cervical premalignant lesions in ThinPrep smears,and search for the identification of specific biomarkers for dysplastic epithelial cells of the cervix as a new examine ways. Methods Random sample 71 patients to be examined tissue test under the colpomicroscope.RT-PCR was used to detect the mRNA of HPV16 E7 and Brn-3a in 71 ThinPrep liquid-based smears specimens. Results In cytologic and pathologic diagnose grades;the positive rates ...
Objective To examine the potential of HPV16 E7 and Brn-3a as a biomarker for cervical premalignant lesions in ThinPrep smears,and search for the identification of specific biomarkers for dysplastic epithelial cells of the cervix as a new examine ways. Methods Random sample 71 patients to be examined tissue test under the colpomicroscope.RT-PCR was used to detect the mRNA of HPV16 E7 and Brn-3a in 71 ThinPrep liquid-based smears specimens. Results In cytologic and pathologic diagnose grades;the positive rates ...
2006, 33(08): 572-574.
DOI: 10.3971/j.issn.1000-8578.1826
Abstract：
Objective To study the relation between polymorphisms of N 5,10 -methylenetetrahydrofolate reductase(MTHFR) and susceptibility of lung cancer. Methods We conducted a case control study with 100 cases of lung cancer and 100 population based controls in Guangdong province. MTHFR genotypes were detected by PCR-RFLP method. Results The expressions of MTHFR genotypes have a statistically significant difference between lung cancer and healthy people(P= 0.030). And there are on such difference among different gender...
Objective To study the relation between polymorphisms of N 5,10 -methylenetetrahydrofolate reductase(MTHFR) and susceptibility of lung cancer. Methods We conducted a case control study with 100 cases of lung cancer and 100 population based controls in Guangdong province. MTHFR genotypes were detected by PCR-RFLP method. Results The expressions of MTHFR genotypes have a statistically significant difference between lung cancer and healthy people(P= 0.030). And there are on such difference among different gender...
2006, 33(08): 575-577.
DOI: 10.3971/j.issn.1000-8578.1827
Abstract：
Objective We studied the association between the expression of epidermal growth factor receptor(EGFR), Cyclooxygenase-2 (COX-2), microvessel density(MVD) and disease free survival in early stage non-small cell lung cancer (NSCLC). Methods Thirty-six patients with NSCLC undergoing complete surgical resection were evaluated retrospectively. Tumor specimens were stained for EGFR, COX-2 and specific MVD marker(CD34) by Immunohistochemistry. Results EGFR and COX-2 over expressions were demonstrated in 44% and 53% ...
Objective We studied the association between the expression of epidermal growth factor receptor(EGFR), Cyclooxygenase-2 (COX-2), microvessel density(MVD) and disease free survival in early stage non-small cell lung cancer (NSCLC). Methods Thirty-six patients with NSCLC undergoing complete surgical resection were evaluated retrospectively. Tumor specimens were stained for EGFR, COX-2 and specific MVD marker(CD34) by Immunohistochemistry. Results EGFR and COX-2 over expressions were demonstrated in 44% and 53% ...
2006, 33(08): 578-580.
DOI: 10.3971/j.issn.1000-8578.1824
Abstract：
Objective To investigate the expressions of SDF-1, CXCR4,and their clinical significance in non-small cell lung cancer. Methods SDF-1,CXCR4 were detected in 40 NSCLC tissues and 10 control group lung tissues by immunohistochemistry. Results In NSCLC tissues, positive rate of SDF-1,CXCR4 was 65.00%(26/40), 45.00%(18/40) respectively. There was significant difference in positive rate of SDF-1 and CXCR4, as compared to normal lung tissue(P< 0.01,P< 0.05). Expressions of SDF-1 and CXCR4 were closely associ...
Objective To investigate the expressions of SDF-1, CXCR4,and their clinical significance in non-small cell lung cancer. Methods SDF-1,CXCR4 were detected in 40 NSCLC tissues and 10 control group lung tissues by immunohistochemistry. Results In NSCLC tissues, positive rate of SDF-1,CXCR4 was 65.00%(26/40), 45.00%(18/40) respectively. There was significant difference in positive rate of SDF-1 and CXCR4, as compared to normal lung tissue(P< 0.01,P< 0.05). Expressions of SDF-1 and CXCR4 were closely associ...
2006, 33(08): 581-582.
DOI: 10.3971/j.issn.1000-8578.1828
Abstract：
Objective This study was designed to investigate the relationship between Podoplanin and VEGF-C in human colon carcinomas and discuss the significance of Podoplanin、VEGF-C in lymph mode metastasis. Methods The expression of Podoplanin and VEGF-C in forty-eight colon carcinoma tissues and ten adjacent noncancerous specimens was evaluated by immunohistochemical analysis. Results (1) Podoplanin was overexpressed in 27 of 48 carcinoma tissues ( 56.3%) and VEGF-C was overexpressed in 31 of 48 ( 64.6%),which were s...
Objective This study was designed to investigate the relationship between Podoplanin and VEGF-C in human colon carcinomas and discuss the significance of Podoplanin、VEGF-C in lymph mode metastasis. Methods The expression of Podoplanin and VEGF-C in forty-eight colon carcinoma tissues and ten adjacent noncancerous specimens was evaluated by immunohistochemical analysis. Results (1) Podoplanin was overexpressed in 27 of 48 carcinoma tissues ( 56.3%) and VEGF-C was overexpressed in 31 of 48 ( 64.6%),which were s...
2006, 33(08): 583-584.
DOI: 10.3971/j.issn.1000-8578.1825
Abstract：
Objective To explore the value of serum tumor markers in predicting bone metastases of breast cancer. Methods Serum tumor markers (CA153、CA125、CEA、FER ) were assayed with CLIA and whole body bone scan were investigated with SPECT on 174 breast cancer patients. Results Of 174 patients,55 were diagnosed as bone metastases with whole body bone scan.The positive predicting values of CA153、CA125、CEA、FER were 68.0%(34/50),70.0%(14/20),88.9%(8/9),53.8%(14/26),and the negative predicting values of CA153、CA125、CEA、FER...
Objective To explore the value of serum tumor markers in predicting bone metastases of breast cancer. Methods Serum tumor markers (CA153、CA125、CEA、FER ) were assayed with CLIA and whole body bone scan were investigated with SPECT on 174 breast cancer patients. Results Of 174 patients,55 were diagnosed as bone metastases with whole body bone scan.The positive predicting values of CA153、CA125、CEA、FER were 68.0%(34/50),70.0%(14/20),88.9%(8/9),53.8%(14/26),and the negative predicting values of CA153、CA125、CEA、FER...
2006, 33(08): 585-587.
DOI: 10.3971/j.issn.1000-8578.1822
Abstract：
Objective To evaluate the diagnostic and therapeutic value of serum CA125,CA19-9 and CEA in colorectal carcinoma. Methods A total of 82 patients with colorectal carcinoma had been treated surgically between June 2004 and June 2005.The control group included 41 healthy adults.The serum CA125,CA19-9 and CEA are determined by protein chip. Results The levels of serum CA125,CA19-9 were higher in the colorectal cancer group than in the control group.The positive rates of serum CA125,CA19-9 and CEA were not associa...
Objective To evaluate the diagnostic and therapeutic value of serum CA125,CA19-9 and CEA in colorectal carcinoma. Methods A total of 82 patients with colorectal carcinoma had been treated surgically between June 2004 and June 2005.The control group included 41 healthy adults.The serum CA125,CA19-9 and CEA are determined by protein chip. Results The levels of serum CA125,CA19-9 were higher in the colorectal cancer group than in the control group.The positive rates of serum CA125,CA19-9 and CEA were not associa...
2006, 33(08): 588-590.
DOI: 10.3971/j.issn.1000-8578.1823
Abstract：
Objective To study relative lymphocyte count and thromboplastid count in peripheral blood and their relationship with prognosis of NSCLC patients. Methods To analyze these two counts and survival time and their relationship with prognosis by χ 2 test and Kaplan-Meier curves. Results The median survival time in the group of relative lymphocyte count ≤20% is 2 months, that in the other group is 6 months.The median survival time in the group of thromboplastid count ≤250×10 9/L is 4 months,that in the other group...
Objective To study relative lymphocyte count and thromboplastid count in peripheral blood and their relationship with prognosis of NSCLC patients. Methods To analyze these two counts and survival time and their relationship with prognosis by χ 2 test and Kaplan-Meier curves. Results The median survival time in the group of relative lymphocyte count ≤20% is 2 months, that in the other group is 6 months.The median survival time in the group of thromboplastid count ≤250×10 9/L is 4 months,that in the other group...
2006, 33(08): 591-592.
DOI: 10.3971/j.issn.1000-8578.1347
Abstract：
Objective To investigate the useless of TAI on breast cancer before the operation. Methods 15 patients of breast cancer confirmed by pathology,in all of them the interventional therapy was performed by using Seldinger' s technique,catheterial head was ultraselectly sent into tumor s feeding artery,chemotherapic protocol was 2 or 3 combination of cisplatinum,adriamycin,5-Fu,Mitoxantrone,pirarubicill.If necessary,gelform can be used for the embolization. Results In all 15 patients after the interventional thera...
Objective To investigate the useless of TAI on breast cancer before the operation. Methods 15 patients of breast cancer confirmed by pathology,in all of them the interventional therapy was performed by using Seldinger' s technique,catheterial head was ultraselectly sent into tumor s feeding artery,chemotherapic protocol was 2 or 3 combination of cisplatinum,adriamycin,5-Fu,Mitoxantrone,pirarubicill.If necessary,gelform can be used for the embolization. Results In all 15 patients after the interventional thera...
2006, 33(08): 593-595.
DOI: 10.3971/j.issn.1000-8578.1344
Abstract：
Objective The objective of this article is to evaluate the response,toxicity and survival time of NO regimenOxaliplation(LOHP)plusvinorelbine(NVB) in the treatment of advanced NSCLC. Methods A total of 77 cases with advanced NSCLC were included in study.All patients were received NVB 25mg/m2d1andd8 and LOHP \ every 3~4 weeks.The whole course of treatment consisted at least 2 cycles. Results The response and the clinical benefit response rates(CBRs) were 33.78% and 77.03% respectively.The re...
Objective The objective of this article is to evaluate the response,toxicity and survival time of NO regimenOxaliplation(LOHP)plusvinorelbine(NVB) in the treatment of advanced NSCLC. Methods A total of 77 cases with advanced NSCLC were included in study.All patients were received NVB 25mg/m2d1andd8 and LOHP \ every 3~4 weeks.The whole course of treatment consisted at least 2 cycles. Results The response and the clinical benefit response rates(CBRs) were 33.78% and 77.03% respectively.The re...
2006, 33(08): 596-598.
DOI: 10.3971/j.issn.1000-8578.1345
Abstract：
Objective To evaluate the therapeutic effectiveness and adverseevents of concurrent gemcitabine chemotherapy and 3-dimentional conformalradiotherapy (3D-CRT) for elderly advanced non-small cell lung cancer(NSCLC). Methods 65 patients of stage Ⅲ NSCLC were enrolled into twogroups: 35cases in the treatment group received concurrent chemotherapy(gemcitabine 1000mg/m2/w, continuous 3 weeks) and 3D-CRT: were given,(3~4)Gyper fractions,5 fraction a week,to total dose of (40~52) Gy/(3~4) week.30 casesin the control...
Objective To evaluate the therapeutic effectiveness and adverseevents of concurrent gemcitabine chemotherapy and 3-dimentional conformalradiotherapy (3D-CRT) for elderly advanced non-small cell lung cancer(NSCLC). Methods 65 patients of stage Ⅲ NSCLC were enrolled into twogroups: 35cases in the treatment group received concurrent chemotherapy(gemcitabine 1000mg/m2/w, continuous 3 weeks) and 3D-CRT: were given,(3~4)Gyper fractions,5 fraction a week,to total dose of (40~52) Gy/(3~4) week.30 casesin the control...
2006, 33(08): 599-601.
DOI: 10.3971/j.issn.1000-8578.1342
Abstract：
Objective To compare the treatment plans between X-ray comformal radiotherapy and proton radiation therapy (PRT) for patients with medulloblastoma,to obtain a better technique in the treatment of medulloblastoma. Methods Five patients with medulloblastoma were chosen in the study.Using the Eclipse photon 7.3.2 and Eclipse proton 7.3.2 treatment planning systems from Varian,two different methods of radiotherapy planning: X-ray conformal planning and proton conformal planning were designed for the patients.The...
Objective To compare the treatment plans between X-ray comformal radiotherapy and proton radiation therapy (PRT) for patients with medulloblastoma,to obtain a better technique in the treatment of medulloblastoma. Methods Five patients with medulloblastoma were chosen in the study.Using the Eclipse photon 7.3.2 and Eclipse proton 7.3.2 treatment planning systems from Varian,two different methods of radiotherapy planning: X-ray conformal planning and proton conformal planning were designed for the patients.The...
2006, 33(08): 602-604.
DOI: 10.3971/j.issn.1000-8578.1343
Abstract：
Objective To explore the prognostic importance of hypoxia inducible factor-1α(HIF-1α)expression in esophageal squamous cell carcinoma (ESCC). Methods The expression of HIF-1α was detected by immunohistochemical staining (SP)in fifty patients with ESCC received radiatherapy. Results HIF-1α was expressed in the nucleus of tumor cells in 34/50 (68%) cases with ESCC. The complete effective rate to radiotherapy and median survival periods and 95 confidence interval of those with positive expression of HIF-1α and...
Objective To explore the prognostic importance of hypoxia inducible factor-1α(HIF-1α)expression in esophageal squamous cell carcinoma (ESCC). Methods The expression of HIF-1α was detected by immunohistochemical staining (SP)in fifty patients with ESCC received radiatherapy. Results HIF-1α was expressed in the nucleus of tumor cells in 34/50 (68%) cases with ESCC. The complete effective rate to radiotherapy and median survival periods and 95 confidence interval of those with positive expression of HIF-1α and...
2006, 33(08): 605-608.
DOI: 10.3971/j.issn.1000-8578.1341
Abstract：
Objective To observe and compare the effects of inhalational combined intravenous anesthesia and epidural general anesthesia on liver function in patients undergoing liver cancer. Methods 30 patients with ASA status Ⅰ～Ⅱ Child-pugh A undergoing hepatolobectomy were randomly divided into two groups: group Ⅰ,united group (n=15); group Ⅱ, control group (n=15). Patients in group ⅠT_ 8～9 were exposed to 0.2% ropivacaine 5ml until testing satisfied. Then followed tracheal intubation, and thetotal is 10ml. Patients...
Objective To observe and compare the effects of inhalational combined intravenous anesthesia and epidural general anesthesia on liver function in patients undergoing liver cancer. Methods 30 patients with ASA status Ⅰ～Ⅱ Child-pugh A undergoing hepatolobectomy were randomly divided into two groups: group Ⅰ,united group (n=15); group Ⅱ, control group (n=15). Patients in group ⅠT_ 8～9 were exposed to 0.2% ropivacaine 5ml until testing satisfied. Then followed tracheal intubation, and thetotal is 10ml. Patients...
