1999 Vol. 26 No. 4
1999, 26(4): 241-243.
Abstract：
Objective: To observe effect of interferon alpha on cell growth and cell cycles of human endometrial adenocarcinoma cell line (JEC) established in our laboratory . Methods Effect of cell growth curve and lectin receptors on the cells were detected after incubation with different concentration at 2000u/ml, 500u/ml, 100u/ml of interferon alpha for 7 days, and alteration of cell cycle were analysed using flow cytometry. Results and Conclusion: Interferon alpha can inhibit the proliferation of JEC and the growth of the cells were dose-dependently suppressed. There were no changes in expression of lectin receptors. The cells showed G1/G2 and Sincrese, but G2/M arrest, it was positive correlation between the former and the concentration of interferon alpha, however, the two latters were negative correlation.
Objective: To observe effect of interferon alpha on cell growth and cell cycles of human endometrial adenocarcinoma cell line (JEC) established in our laboratory . Methods Effect of cell growth curve and lectin receptors on the cells were detected after incubation with different concentration at 2000u/ml, 500u/ml, 100u/ml of interferon alpha for 7 days, and alteration of cell cycle were analysed using flow cytometry. Results and Conclusion: Interferon alpha can inhibit the proliferation of JEC and the growth of the cells were dose-dependently suppressed. There were no changes in expression of lectin receptors. The cells showed G1/G2 and Sincrese, but G2/M arrest, it was positive correlation between the former and the concentration of interferon alpha, however, the two latters were negative correlation.
1999, 26(4): 244-246.
Abstract：
Objective: To study the levels of plasma endothelin and its effect on fibrinolysis in acuteleukemia, and its roles in developing and bleeding of leukemia. Methods: Plasma endothelin(ET)was measured with radioimmunoassay. Plasminogen activity was measured with aceta lysis chromogenesis. Tissue-type plasminogen activator activity and plasminogen activator inhibitor activity were measured with chromogenic substrates. Ddimer was measured with ELISA. Results: The levels of ET were higher in acute leukemia than those in normal controls, especially in primary and non-remission acute leukemia markedly higher, in the groups of complete remission were lower, but higher than those in the controls. The levels of ET were higher in leukemia with bleeding than those in without bleeding .ET and tissue-type plasminogen activator activity had negative correlation. Conclusion: ET not only had important value in diagonosis and prognosis of leukemia, but also had relation with bleeding in acute leukemia.The correlation of ET and tissue-type plasminogen activator activity indicated ET might play a role in regulating fibrinolysis serving as an antifibrinolysis factor.
Objective: To study the levels of plasma endothelin and its effect on fibrinolysis in acuteleukemia, and its roles in developing and bleeding of leukemia. Methods: Plasma endothelin(ET)was measured with radioimmunoassay. Plasminogen activity was measured with aceta lysis chromogenesis. Tissue-type plasminogen activator activity and plasminogen activator inhibitor activity were measured with chromogenic substrates. Ddimer was measured with ELISA. Results: The levels of ET were higher in acute leukemia than those in normal controls, especially in primary and non-remission acute leukemia markedly higher, in the groups of complete remission were lower, but higher than those in the controls. The levels of ET were higher in leukemia with bleeding than those in without bleeding .ET and tissue-type plasminogen activator activity had negative correlation. Conclusion: ET not only had important value in diagonosis and prognosis of leukemia, but also had relation with bleeding in acute leukemia.The correlation of ET and tissue-type plasminogen activator activity indicated ET might play a role in regulating fibrinolysis serving as an antifibrinolysis factor.
1999, 26(4): 247-249.
Abstract：
Objective: In order to investigate the relationship between Helicobacter Pylori (Hp)infection and gastric cancer. Methods: A 1:1 matched casecontrol study was conducted in Changle, which is one of gastric cancer highest mortality in the world. Results: The Hp infection rate was 63.68 % in all subjects. No significant difference (P >0.05) was seen between cases and controls. Hp infection rates were 68.18 % in gastric adenocarcinoma cases, and 56.00 % in gastric cardia cancer cases. The prevalence of Hp was high in the risk areas of gastric cancer. Conclusion: The combined effect between Hp infection and other risk factors (fish sauce, less consumption of fresh vegetables, bad dietary habits) played an important role in gastric cancer.
Objective: In order to investigate the relationship between Helicobacter Pylori (Hp)infection and gastric cancer. Methods: A 1:1 matched casecontrol study was conducted in Changle, which is one of gastric cancer highest mortality in the world. Results: The Hp infection rate was 63.68 % in all subjects. No significant difference (P >0.05) was seen between cases and controls. Hp infection rates were 68.18 % in gastric adenocarcinoma cases, and 56.00 % in gastric cardia cancer cases. The prevalence of Hp was high in the risk areas of gastric cancer. Conclusion: The combined effect between Hp infection and other risk factors (fish sauce, less consumption of fresh vegetables, bad dietary habits) played an important role in gastric cancer.
1999, 26(4): 250-251.
Abstract：
According to that the blood group substance in saliva can specifically combine to it's corresponding antibody, the method of neutralization test was adopted to determine the differences in the concentration of the blood groop substance between 60 cases of healthy people whose blood types were known and 30 cases of patients with carcinoma of stomach, as well as the differences after and before operation in patients with gastric cancer, and also between patients with poorly differentiated gastric adenocarcinoma and patients with other pathologic types. The result was statistically analysised. The concentration of A type and H type blood group substance in patients with gastric cancer elevated significantly as contrast to those in healthy people (A type, P <0.01; O type, P <0.05). The concentration of blood group substance in patients of A blood type but not in patients of B or O blood type decreased obviously. The result indicates that concentrationes of A and H blood group substance in patients with poorly differentiated adenocarcinoma elevated significantly as compare to those in patients with the other pathologic types (P <0.01). All the results suggest that determination of the blood group substance can be used as an auxiliary index of diagnosing the gastric cancer and also as a prognostic indicator for the patients of A and O blood type.
According to that the blood group substance in saliva can specifically combine to it's corresponding antibody, the method of neutralization test was adopted to determine the differences in the concentration of the blood groop substance between 60 cases of healthy people whose blood types were known and 30 cases of patients with carcinoma of stomach, as well as the differences after and before operation in patients with gastric cancer, and also between patients with poorly differentiated gastric adenocarcinoma and patients with other pathologic types. The result was statistically analysised. The concentration of A type and H type blood group substance in patients with gastric cancer elevated significantly as contrast to those in healthy people (A type, P <0.01; O type, P <0.05). The concentration of blood group substance in patients of A blood type but not in patients of B or O blood type decreased obviously. The result indicates that concentrationes of A and H blood group substance in patients with poorly differentiated adenocarcinoma elevated significantly as compare to those in patients with the other pathologic types (P <0.01). All the results suggest that determination of the blood group substance can be used as an auxiliary index of diagnosing the gastric cancer and also as a prognostic indicator for the patients of A and O blood type.
1999, 26(4): 252-254.
Abstract：
Objective: To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity. Methods: Treated with 10-6 mol/L all-trans retinoic acid(ATRA), gastric cancer cells, BGC-823 and MKN-45, were measured by flow cytometry for cell cycle distribution, by microplate reader for enzyme activity, by 3H-TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate. Results: After treated with ATRA, BGC-823 was lead to stay in G0/G1 phase, while LDH, ALP and β-G activity was suppressed, the inhibition rates were 22.40 %, 32.94 % and 41.35 %, respectively. DNA synthetic speed was decreased by 41.43 % and growth inhibition rate was 61.03 %. Nevertheless, MKN-45 can't be changed as BGC-823. Conclusion: BGC-823, but not MKN-45, can be induced differentiation and cell growth can be suppressed by ATRA.
Objective: To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity. Methods: Treated with 10-6 mol/L all-trans retinoic acid(ATRA), gastric cancer cells, BGC-823 and MKN-45, were measured by flow cytometry for cell cycle distribution, by microplate reader for enzyme activity, by 3H-TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate. Results: After treated with ATRA, BGC-823 was lead to stay in G0/G1 phase, while LDH, ALP and β-G activity was suppressed, the inhibition rates were 22.40 %, 32.94 % and 41.35 %, respectively. DNA synthetic speed was decreased by 41.43 % and growth inhibition rate was 61.03 %. Nevertheless, MKN-45 can't be changed as BGC-823. Conclusion: BGC-823, but not MKN-45, can be induced differentiation and cell growth can be suppressed by ATRA.
1999, 26(4): 255-257.
Abstract：
Objective: To study the radiosensitivity of human gastric cancer cell line(SGC-7901)and two subcell lines picked from the line. Methods: The cells of the cell line and two subcell lines (large clone and small clone) were radioted at different doses with 8MeV linear accelerator. Then the cells were incubated for 48h, 72h and clone culture. The survival curve was established by counting cell. Results: (1)The values of Do between two subcell lines were significantly different(P <0.05); (2)the values of Do between large clone subcell line and SGC-7901 cell line were significantly different (P <0.05). Conclusion: Heterogeneity of the radiosensitivity among SGC-7901 and the subcell lines is suggested.
Objective: To study the radiosensitivity of human gastric cancer cell line(SGC-7901)and two subcell lines picked from the line. Methods: The cells of the cell line and two subcell lines (large clone and small clone) were radioted at different doses with 8MeV linear accelerator. Then the cells were incubated for 48h, 72h and clone culture. The survival curve was established by counting cell. Results: (1)The values of Do between two subcell lines were significantly different(P <0.05); (2)the values of Do between large clone subcell line and SGC-7901 cell line were significantly different (P <0.05). Conclusion: Heterogeneity of the radiosensitivity among SGC-7901 and the subcell lines is suggested.
1999, 26(4): 258-259.
Abstract：
To detect the telomerase activity in human tumor cell line, we established the new method of sliver staining Telomeric Repeat Amplification Protocol(TRAP). We detected the telomerase activity in human tumor cell line and heat-treated cells as control by combining the sliver stain with TRAP. The human tumor cells were positive for the telomerase activity and the heat-treated cells were negative. Comparing with radioisotopic TRAP, sliver staining TRAP was specific, sensitive, harmless and fast assay for telomerase activity. It could be one of the marks in cancer.
To detect the telomerase activity in human tumor cell line, we established the new method of sliver staining Telomeric Repeat Amplification Protocol(TRAP). We detected the telomerase activity in human tumor cell line and heat-treated cells as control by combining the sliver stain with TRAP. The human tumor cells were positive for the telomerase activity and the heat-treated cells were negative. Comparing with radioisotopic TRAP, sliver staining TRAP was specific, sensitive, harmless and fast assay for telomerase activity. It could be one of the marks in cancer.
1999, 26(4): 260-262.
Abstract：
B16 Melanoma cells were inoculated into the BALB/C mice to establish melanoma-bearing models. The antitumor effect of Tween-80 in combination with hyperthermia 41°C was studied. We observed the changes of the mortality of tumor-bearing mice, the tumor growth curves, activities of serum tumor necrosis factor (sTNF) and the level of serum sialic acid (sSA) in tumor-bearing mice. The number of pulmonary metastatic tumor foci from blood flow was also detected. The results showed that combined with Tween-80, hyperthermia 41°C could dramatically suppress the growth of the melanoma in the feet of mice, survive the tumor-bearing mice and decrease the number of pulmonary metastatic tumor foci but no significant effects were observed by treatment with Tween-80 or hyperthermia at 41°C alone. The activities of sTNF and the level of sSA of the melanoma-bearing mice kept at higher levels than those of normal BALB/C mice. Tween-80 combined with heating 41°C significantly decreased the activities of sTNF and increased the level of sSA that decreased 10 weeks later with the tumor regression. These results demonstrate that Tween-80 may make hyperthermia exert effective antitumor effect below the critical temperature and increase the safety of hyperthermia in treatment. It is one of the most ideal synergist with hyperthermia. The changes of sTNF and sSA suggest that the synergetic effect could involve in facilitating the exposure of tumor antigen and activation of immune system.
B16 Melanoma cells were inoculated into the BALB/C mice to establish melanoma-bearing models. The antitumor effect of Tween-80 in combination with hyperthermia 41°C was studied. We observed the changes of the mortality of tumor-bearing mice, the tumor growth curves, activities of serum tumor necrosis factor (sTNF) and the level of serum sialic acid (sSA) in tumor-bearing mice. The number of pulmonary metastatic tumor foci from blood flow was also detected. The results showed that combined with Tween-80, hyperthermia 41°C could dramatically suppress the growth of the melanoma in the feet of mice, survive the tumor-bearing mice and decrease the number of pulmonary metastatic tumor foci but no significant effects were observed by treatment with Tween-80 or hyperthermia at 41°C alone. The activities of sTNF and the level of sSA of the melanoma-bearing mice kept at higher levels than those of normal BALB/C mice. Tween-80 combined with heating 41°C significantly decreased the activities of sTNF and increased the level of sSA that decreased 10 weeks later with the tumor regression. These results demonstrate that Tween-80 may make hyperthermia exert effective antitumor effect below the critical temperature and increase the safety of hyperthermia in treatment. It is one of the most ideal synergist with hyperthermia. The changes of sTNF and sSA suggest that the synergetic effect could involve in facilitating the exposure of tumor antigen and activation of immune system.
1999, 26(4): 263-264.
Abstract：
To observe the effect of cyto laser on immulogical mechanism. Methods: Double antibody sandwith enzyme linked immunosorbent assay( ELISA) and MIT test were used to measure the effect of laser on IL6, IL8 and IL1 tumor-bearing mice, Results: When laser acted on spleen region, promoting IL-1 and IL-8 production and suppressing IL-6 production, compared with control group showed very significant changes ( P<0.01) Conclusion: Laser has regulating effect of interleukin production and antitumor action.
To observe the effect of cyto laser on immulogical mechanism. Methods: Double antibody sandwith enzyme linked immunosorbent assay( ELISA) and MIT test were used to measure the effect of laser on IL6, IL8 and IL1 tumor-bearing mice, Results: When laser acted on spleen region, promoting IL-1 and IL-8 production and suppressing IL-6 production, compared with control group showed very significant changes ( P<0.01) Conclusion: Laser has regulating effect of interleukin production and antitumor action.
1999, 26(4): 265-267.
Abstract：
Objective: To evaluate the significance of p21, p185, EGFR protein expression and LN, type IV collagen in human osteosarcoma . Methods: p21, p185, EGFR protein expression and LN, type IV collagen were examined in 40 cases human osteosarcoma tissue with immunohistochemical ABC methods. Results: Accumulation of p21 protein was found in 8 out of 38 cases osteosarcomas. The positive rates of p185 andEGFR were respectively 65.8 % and 50.0 %. It was observed that the majority of LN and type IV collagen expression in osteosarcoma was defective. Conclusion: The results of p21, p185 and EGFR high expression in human osteosarcoma suggest that they did have a correlation with osteosarcoma origin and development; The changes of LN and type IV collagen have significant effect on osteosarcoma invade and metastasis.
Objective: To evaluate the significance of p21, p185, EGFR protein expression and LN, type IV collagen in human osteosarcoma . Methods: p21, p185, EGFR protein expression and LN, type IV collagen were examined in 40 cases human osteosarcoma tissue with immunohistochemical ABC methods. Results: Accumulation of p21 protein was found in 8 out of 38 cases osteosarcomas. The positive rates of p185 andEGFR were respectively 65.8 % and 50.0 %. It was observed that the majority of LN and type IV collagen expression in osteosarcoma was defective. Conclusion: The results of p21, p185 and EGFR high expression in human osteosarcoma suggest that they did have a correlation with osteosarcoma origin and development; The changes of LN and type IV collagen have significant effect on osteosarcoma invade and metastasis.
1999, 26(4): 268-269.
Abstract：
Objective： Observing the ralationship between HBV and liver damage in chemotherapy of Lymphoma. Methods： Among 182 cases of lymphoma, 53 cases with positive serum HBV markerwhile 129 cases without HBV marker. The liver funtion of all cases were normal before chemotherapy, effort was made to observe the patients liver function damage after chemotherapy. Results： After two phasesregular chemotherapy, the liver damage was 45.3 % (24/53) in HBV(+) group and 27.1 % (35/129) in HBV(-)group (P <0.05). Furthermore, among the patients in HBV(+)group, 10 cases were treated with Interferon alfa -2b and the others didnt use the drug . Liver function damage was 10.0 %(1/10) in the former while 53.5 %(24/43) in the latter (P <0.05). Conclusion： The cases with HBV(+) were more likely to appear liver function damage, which could be alleviated by using Interferon alfa -2b.
Objective： Observing the ralationship between HBV and liver damage in chemotherapy of Lymphoma. Methods： Among 182 cases of lymphoma, 53 cases with positive serum HBV markerwhile 129 cases without HBV marker. The liver funtion of all cases were normal before chemotherapy, effort was made to observe the patients liver function damage after chemotherapy. Results： After two phasesregular chemotherapy, the liver damage was 45.3 % (24/53) in HBV(+) group and 27.1 % (35/129) in HBV(-)group (P <0.05). Furthermore, among the patients in HBV(+)group, 10 cases were treated with Interferon alfa -2b and the others didnt use the drug . Liver function damage was 10.0 %(1/10) in the former while 53.5 %(24/43) in the latter (P <0.05). Conclusion： The cases with HBV(+) were more likely to appear liver function damage, which could be alleviated by using Interferon alfa -2b.
1999, 26(4): 270-271.
Abstract：
Objective: To explore the relation between the Gene Mutation of P53 and Prognosis in pediatric Non-Hodgkin Lymphoma. Methods: To detect the 5 -6 exon of P53 gene in 31 Ⅱ-Ⅲ phases pediatric Non-Hodgkin Lymphoma patients by PCR-SSCP technique and follow-up study them for 8 years. Results: The gene mutation of P53were found in 11 children (11/31)and the recurrecewere observed in 7 of them. The recurrece was only observed in 1 patient of 20 children without gene mutation of P53. Conclusion: The gene mutation of P53 is one of the indicators of prognosis in pediatric Non-Hodgkin Lymphoma and is related with recurrence in early stage.
Objective: To explore the relation between the Gene Mutation of P53 and Prognosis in pediatric Non-Hodgkin Lymphoma. Methods: To detect the 5 -6 exon of P53 gene in 31 Ⅱ-Ⅲ phases pediatric Non-Hodgkin Lymphoma patients by PCR-SSCP technique and follow-up study them for 8 years. Results: The gene mutation of P53were found in 11 children (11/31)and the recurrecewere observed in 7 of them. The recurrece was only observed in 1 patient of 20 children without gene mutation of P53. Conclusion: The gene mutation of P53 is one of the indicators of prognosis in pediatric Non-Hodgkin Lymphoma and is related with recurrence in early stage.
1999, 26(4): 272-274.
Abstract：
Objective: To determine the relation of microvessel density (MVD)to the prognosis and the clinicopathological factors in axillary-node-negative breast cancer (ANNBC). Methods: MVD in 120 cases paraffin specimens of ANNBC. were examinated using imminohistochemistry technique. The relationship between MVD and overall survival (OS), relapsefree survival (RFS), metastasis-free survival (MFS) were analysed, respectively, as well as it was done between MVD and five clinicopathological factors. Results: The mean MVD was 38.21 ±18.72. It didn't relate to those clinicopathological factors. MVD in relapse and metastasis group which was apparently higher than that in desease-free group; OS, RFS, MFS curve in higher MVD group (MD >38) were significiantly lower those that in lower MVD group (MVD ≤38), respectively ; COX model indicated that MVD was an independent prognostic factor in ANNBC. Conclusion: MVD may be a good prognostic indicator for patients with ANNBC.
Objective: To determine the relation of microvessel density (MVD)to the prognosis and the clinicopathological factors in axillary-node-negative breast cancer (ANNBC). Methods: MVD in 120 cases paraffin specimens of ANNBC. were examinated using imminohistochemistry technique. The relationship between MVD and overall survival (OS), relapsefree survival (RFS), metastasis-free survival (MFS) were analysed, respectively, as well as it was done between MVD and five clinicopathological factors. Results: The mean MVD was 38.21 ±18.72. It didn't relate to those clinicopathological factors. MVD in relapse and metastasis group which was apparently higher than that in desease-free group; OS, RFS, MFS curve in higher MVD group (MD >38) were significiantly lower those that in lower MVD group (MVD ≤38), respectively ; COX model indicated that MVD was an independent prognostic factor in ANNBC. Conclusion: MVD may be a good prognostic indicator for patients with ANNBC.
1999, 26(4): 275-277.
Abstract：
Objective: To evaluate the relation between serum copper and serum ferritin in cancer patients. Methods: The levels of serum copper, ferritin in 27 patients with lung cancer and 45 normal subjects were determined. Results: The results showed that the levels of serum copper of lung cancer patients were higher than the healthy controls (P <0.01). The levels of serum ferritin of lung cancer patients were higher than the healthy controls too (P<0.01). Using correlativity analysis, the highly positive correlation was found between serum copper and serum ferritin (P<0.005). Conclusion: The results suggest that higher serum ferritin levels in the lung cancer patients may be due to higher levels of ceruloplasmin.
Objective: To evaluate the relation between serum copper and serum ferritin in cancer patients. Methods: The levels of serum copper, ferritin in 27 patients with lung cancer and 45 normal subjects were determined. Results: The results showed that the levels of serum copper of lung cancer patients were higher than the healthy controls (P <0.01). The levels of serum ferritin of lung cancer patients were higher than the healthy controls too (P<0.01). Using correlativity analysis, the highly positive correlation was found between serum copper and serum ferritin (P<0.005). Conclusion: The results suggest that higher serum ferritin levels in the lung cancer patients may be due to higher levels of ceruloplasmin.
1999, 26(4): 278-280.
Abstract：
Objective: To evaluat the value of measurement of 4 tumor markers (CEA, CA50, CA19-9, CA125) for diagnosis and evaluation of disease extent in patients with lung cancer. Methods: These tumor markers in serum were measured in 62 patients with lung cancers, and change of the markers were observed in 58 patients with different stage and after or before treatment. Results: Combination of these tumor markers increased sensitivity and accuracy of measurement, the most offective combination seemed to be CEA puls CA50 and CA19-9. The serum levels of the markers in patients with advanced disease (stage Ⅲ and Ⅳ) were significantly higher than in those with stage Ⅰ and Ⅱ. After surgery and chemotherapy (curative effects :CRPR), the serum levels of the markers in pafients was decrease significantly. Conclusion: Measurement of CEA, CA50 and CA19-9 in serum provide useful informafion for diagnosis and evaluation of disease extent in patients with lung cancer.
Objective: To evaluat the value of measurement of 4 tumor markers (CEA, CA50, CA19-9, CA125) for diagnosis and evaluation of disease extent in patients with lung cancer. Methods: These tumor markers in serum were measured in 62 patients with lung cancers, and change of the markers were observed in 58 patients with different stage and after or before treatment. Results: Combination of these tumor markers increased sensitivity and accuracy of measurement, the most offective combination seemed to be CEA puls CA50 and CA19-9. The serum levels of the markers in patients with advanced disease (stage Ⅲ and Ⅳ) were significantly higher than in those with stage Ⅰ and Ⅱ. After surgery and chemotherapy (curative effects :CRPR), the serum levels of the markers in pafients was decrease significantly. Conclusion: Measurement of CEA, CA50 and CA19-9 in serum provide useful informafion for diagnosis and evaluation of disease extent in patients with lung cancer.
1999, 26(4): 281-283.
Abstract：
Objective: to study the expression of P21.P53 in rectal cancer and its relation to clinical pathological characteristics and the significance of its prognosing rectal cancer. Methods: Using immunohistochemistry (s-p method) P21.P53 were studied in 110 cases of rectal the expression of cancer, using normal rectum as controlling group. Results the positive rate of P21.P53 oncoprotein was 54.5 %、42.7 %, the expression of P53 had no relation to clinical pathological characteristics of rectal cancer, the cases with expressing P21 oncoprotein had lower 3 or 5 year survival rate, worsely differentiated, higher rate of lymph metastasis. Conclusion: P53 gene might be related to rectal. carcinogenisis, P21 might be prognosis of rectalcancer.
Objective: to study the expression of P21.P53 in rectal cancer and its relation to clinical pathological characteristics and the significance of its prognosing rectal cancer. Methods: Using immunohistochemistry (s-p method) P21.P53 were studied in 110 cases of rectal the expression of cancer, using normal rectum as controlling group. Results the positive rate of P21.P53 oncoprotein was 54.5 %、42.7 %, the expression of P53 had no relation to clinical pathological characteristics of rectal cancer, the cases with expressing P21 oncoprotein had lower 3 or 5 year survival rate, worsely differentiated, higher rate of lymph metastasis. Conclusion: P53 gene might be related to rectal. carcinogenisis, P21 might be prognosis of rectalcancer.
1999, 26(4): 284-285.
Abstract：
The contents of Laminin (LN) and Collagen type Ⅳ (Col Ⅳ) in serum were measured by radioimmunoassay in patients with colorectal cancer. It was showed that the content of LN and Col Ⅳ in cancer group was significantly higher than those in control group (P <0.01), the content of LN and Col Ⅳ in group with lymph node metastases was higher than those in group without lymph node metastases (P <0.05). The content of LN in Dukes C group was hight than that in Dukes B group (P <0.05). There was a tendency that the content of serum Col Ⅳ increased with the advance of the Dukes clasification and content of serum Col Ⅳ in DukesD group was higher than that in Dukes B group. This implies that the contents of the serum LN and Col Ⅳ is closely related to the tumor invasion and metastases, and the assay of the LN and Col Ⅳ in serum may be useful to the diagnosis of colorectal cancer.
The contents of Laminin (LN) and Collagen type Ⅳ (Col Ⅳ) in serum were measured by radioimmunoassay in patients with colorectal cancer. It was showed that the content of LN and Col Ⅳ in cancer group was significantly higher than those in control group (P <0.01), the content of LN and Col Ⅳ in group with lymph node metastases was higher than those in group without lymph node metastases (P <0.05). The content of LN in Dukes C group was hight than that in Dukes B group (P <0.05). There was a tendency that the content of serum Col Ⅳ increased with the advance of the Dukes clasification and content of serum Col Ⅳ in DukesD group was higher than that in Dukes B group. This implies that the contents of the serum LN and Col Ⅳ is closely related to the tumor invasion and metastases, and the assay of the LN and Col Ⅳ in serum may be useful to the diagnosis of colorectal cancer.
1999, 26(4): 286-287.
Abstract：
Objective: To investigate the patho-histomorphology featcere of pulmonory-blastomce. Methods: The samples from two patients. accompained with detailed clinical dat.were investignted usihg H.E staining.immunohistochemical assays. Results and Conclusion: There were to see differentiationepithelium composition and undifferentiation mesenchyme tissue in the histomorphology. the latter KT (-)、EMA (-)、、LCA (-)、VM (+). rarelly pulmonory blastoma looks like the embryonal lung blast tissue, which is most commonly seen in children and between the age of thirty and forty years we should distinguish the patho-histomorpho-logy feature from translatic pulmonory blastoma and carci-sarcoma of the lung.
Objective: To investigate the patho-histomorphology featcere of pulmonory-blastomce. Methods: The samples from two patients. accompained with detailed clinical dat.were investignted usihg H.E staining.immunohistochemical assays. Results and Conclusion: There were to see differentiationepithelium composition and undifferentiation mesenchyme tissue in the histomorphology. the latter KT (-)、EMA (-)、、LCA (-)、VM (+). rarelly pulmonory blastoma looks like the embryonal lung blast tissue, which is most commonly seen in children and between the age of thirty and forty years we should distinguish the patho-histomorpho-logy feature from translatic pulmonory blastoma and carci-sarcoma of the lung.
1999, 26(4): 288-289.
Abstract：
Objective: To assess the clinicopathological characteristies and histogogenesis of malignant fibrous histiocytoma of nesal. Methods: Five cases were invesigated at the lighe microscopic level and were studied by immunohistochemical thchniues. Rusults: Three cases were mainly of hemargioma, two cases was of mainly pheomorphic. five cases were prositive in a1 —AACT a1 —AAT Lysozyme by immunohistochemical techniques. Conclusions: Five cases were malignant tumors which came from the histiocytic. The prognosis of tumors was good after surgical treatment combined with rediother apy.
Objective: To assess the clinicopathological characteristies and histogogenesis of malignant fibrous histiocytoma of nesal. Methods: Five cases were invesigated at the lighe microscopic level and were studied by immunohistochemical thchniues. Rusults: Three cases were mainly of hemargioma, two cases was of mainly pheomorphic. five cases were prositive in a1 —AACT a1 —AAT Lysozyme by immunohistochemical techniques. Conclusions: Five cases were malignant tumors which came from the histiocytic. The prognosis of tumors was good after surgical treatment combined with rediother apy.
1999, 26(4): 290-291.
Abstract：
Objective: To study the ultrasonic imaging character and differential diagnosis and pathologic basis of intraocular tumor. Methods: 34 cases of patientsultrasonic imaging character and differential points were reviewing concluded. 30 cases of them have been made pathologic examination after operation. Results: Where there are echoes in ultrasonic pictures, there are rich cells or compact tissues or calcifications, In weak echo place or no echo place, there are few cells, ncrosis, liquefaction or liquid exudation. Conclusion: The ceho intensity of ultrasonic is determined not only by the cellular density, the compact degree of tissues but also by whether threr are calcifications or not. Amount serofluid exudation, necrosis and liquefaction in the tail-like part of the choroid malignant melanoma is the reason for “dig hollow”phenomenon.
Objective: To study the ultrasonic imaging character and differential diagnosis and pathologic basis of intraocular tumor. Methods: 34 cases of patientsultrasonic imaging character and differential points were reviewing concluded. 30 cases of them have been made pathologic examination after operation. Results: Where there are echoes in ultrasonic pictures, there are rich cells or compact tissues or calcifications, In weak echo place or no echo place, there are few cells, ncrosis, liquefaction or liquid exudation. Conclusion: The ceho intensity of ultrasonic is determined not only by the cellular density, the compact degree of tissues but also by whether threr are calcifications or not. Amount serofluid exudation, necrosis and liquefaction in the tail-like part of the choroid malignant melanoma is the reason for “dig hollow”phenomenon.
1999, 26(4): 292-294.
Abstract：
Objective: To evaluate the efficacy and toxicity of domestic rhGM-CSF, Gening in prevention of leucopenia and neutropenia caused by cancer chemotherapy. Methods: A multicenter, randomized, match and cross-over clinical trial, 65 patients were divided into AB and BA groups and two-cycle treatment were given to each patient.In group AB, combination chemotherapy and Gening were given in the first cycle and combination chemotherapy alone was given in the second cycle, while vice versa in group BA. Results: Geningsignificantly increased the number of white blood cell and the absolute neutrophil count at the nadir, shorten the days with leucopenia and neutropenia of chemotherapy patients with the acceptable the adverse reactionseg. mild or moderate fever, injection site reaction, bone pain, myalgia, asthenia and skin rash. Conclusion: Gening is a valuable adjuvant in cancer chemotherapy.
Objective: To evaluate the efficacy and toxicity of domestic rhGM-CSF, Gening in prevention of leucopenia and neutropenia caused by cancer chemotherapy. Methods: A multicenter, randomized, match and cross-over clinical trial, 65 patients were divided into AB and BA groups and two-cycle treatment were given to each patient.In group AB, combination chemotherapy and Gening were given in the first cycle and combination chemotherapy alone was given in the second cycle, while vice versa in group BA. Results: Geningsignificantly increased the number of white blood cell and the absolute neutrophil count at the nadir, shorten the days with leucopenia and neutropenia of chemotherapy patients with the acceptable the adverse reactionseg. mild or moderate fever, injection site reaction, bone pain, myalgia, asthenia and skin rash. Conclusion: Gening is a valuable adjuvant in cancer chemotherapy.
1999, 26(4): 295-296.
Abstract：
Objective: Observe the therapeutic effects and toxicity reaction of MVP regimen for the treatment of non-small cell lung cancer (NSCLC). Methods: 45 cases were treated with MVP (MMC +VDS +DDP) regimen. Results: Response rates were 46.7 % (21/45). Neutropeniawas the dose-limited toxicity and was occurred in 75.6 % of cases. No other serious side reaction occurred in all patients. Conclusions: There was definitive good therapeutic effect but slight side reaction with MVP regimen in the treatment of NSCLC.
Objective: Observe the therapeutic effects and toxicity reaction of MVP regimen for the treatment of non-small cell lung cancer (NSCLC). Methods: 45 cases were treated with MVP (MMC +VDS +DDP) regimen. Results: Response rates were 46.7 % (21/45). Neutropeniawas the dose-limited toxicity and was occurred in 75.6 % of cases. No other serious side reaction occurred in all patients. Conclusions: There was definitive good therapeutic effect but slight side reaction with MVP regimen in the treatment of NSCLC.
1999, 26(4): 297-298.
Abstract：
Objective: To study the effects and toxicities of intraperitoneal combined with intravenous adminstration treatment for advanced-stage cancer in digestive system. Methods: The patients were treated with peri-chemotherapy once every week for two weeks followed by intravenous chemotherapy for two weeks. totalling six weeks as a course of treatment so as to observe the curative effect and the toxicities. Results: Amony 41 cases there were two of complete remission and 24 of partial remission with a total effective rate of 63.4 % all patients showed improved in their symptoms and signs. The toxicities mainly include scleroma at injection site pain in puncturing site. abdominal distension, lypoleucocytosis and mild digective tract reaciton. Conclosion: This treatment is effective for advanced-stage cancer without adbominal dropsy of the digestive system with mild toxicitives thus it can used as a routine treatment.
Objective: To study the effects and toxicities of intraperitoneal combined with intravenous adminstration treatment for advanced-stage cancer in digestive system. Methods: The patients were treated with peri-chemotherapy once every week for two weeks followed by intravenous chemotherapy for two weeks. totalling six weeks as a course of treatment so as to observe the curative effect and the toxicities. Results: Amony 41 cases there were two of complete remission and 24 of partial remission with a total effective rate of 63.4 % all patients showed improved in their symptoms and signs. The toxicities mainly include scleroma at injection site pain in puncturing site. abdominal distension, lypoleucocytosis and mild digective tract reaciton. Conclosion: This treatment is effective for advanced-stage cancer without adbominal dropsy of the digestive system with mild toxicitives thus it can used as a routine treatment.
1999, 26(4): 299-300.
Abstract：
Objective： To explore a new effective regimen for recurrence and distal metasasis carcinoma of the cervix. Methods：Twenty patients with local recurrence and/or distal metastasis carcinoma of the cervix received intraperitoneal chemotherapy were studied. All patients were treated with DDP, 150 mg/m2. The cycle was repeated every 21 days. All patients were evaluable for response. Results： Compared with the other two methods, the tumour shrank obviously after intraperitoned chemotherapy (P<0.001). None of these patients occured serious complication. Conclusion：Our study indicated that intraperitoneal chemotherapy is a effective way for local recurrence and distal metasis carcinoma of the cervix.
Objective： To explore a new effective regimen for recurrence and distal metasasis carcinoma of the cervix. Methods：Twenty patients with local recurrence and/or distal metastasis carcinoma of the cervix received intraperitoneal chemotherapy were studied. All patients were treated with DDP, 150 mg/m2. The cycle was repeated every 21 days. All patients were evaluable for response. Results： Compared with the other two methods, the tumour shrank obviously after intraperitoned chemotherapy (P<0.001). None of these patients occured serious complication. Conclusion：Our study indicated that intraperitoneal chemotherapy is a effective way for local recurrence and distal metasis carcinoma of the cervix.
1999, 26(4): 301-302.
Abstract：
Objective: To evaluate therapeutic effectiveness of postural drainage for esophagomediastinal fistula. Methods: From January 1992 to March 1996, there were 21 patients for esophagomediastinal fistula among 1461 patients for esophageal carcinoma. Fistula position of 21 cases were defined by rotating simulator boom to observe the fistula in barium swallow examination, then according to the fistula position to arrange postural drainage. Results: In one month, chest pain remission 85.7 %(18/21), abatement of fever 77.8 %(7/9), fistula healing 85.7 %(18/21). 3 、6 、12 month survival rates of 21 cases were 90.5 %、66.7 %、33.3 %. Conclusion: Postural drainage could impel esophagomediastinal fistula to heal, influencing factor of esophagomediastinal fistula healing were the shape the length the diameter of the fistula and mediastinitis.
Objective: To evaluate therapeutic effectiveness of postural drainage for esophagomediastinal fistula. Methods: From January 1992 to March 1996, there were 21 patients for esophagomediastinal fistula among 1461 patients for esophageal carcinoma. Fistula position of 21 cases were defined by rotating simulator boom to observe the fistula in barium swallow examination, then according to the fistula position to arrange postural drainage. Results: In one month, chest pain remission 85.7 %(18/21), abatement of fever 77.8 %(7/9), fistula healing 85.7 %(18/21). 3 、6 、12 month survival rates of 21 cases were 90.5 %、66.7 %、33.3 %. Conclusion: Postural drainage could impel esophagomediastinal fistula to heal, influencing factor of esophagomediastinal fistula healing were the shape the length the diameter of the fistula and mediastinitis.
1999, 26(4): 303-304.
Abstract：
Objective: To analysize retrospectively the characteristics of the teenagers with NPC. Methods: 102 teenagers with NPC received external irradiation, among them, 6 cases with N3 diseases was treaed with chemotherapy simutaneously. The tumor dose of primary site was 38Gy-82Gy with an average dose of 66Gy. The number of patients with stage Ⅲ-Ⅳ was 86 which instituted 85% of the whole groupe. Reaults: After a minimum follow-up of 5 years, the overall 5 years survival rate was 58.6%. Administration of RT +CT to 6 cases with N3 disease survived 3 cases. The main cause of deaths was remote metastases. Sterility and secondary amenorrhea was found in this group. Conclusion: (1) Applications of chemotherpy may improve the suvives of patients with N3 disease. (2) The protection of hypophysis of the youngs against irradiation should be emphasized.
Objective: To analysize retrospectively the characteristics of the teenagers with NPC. Methods: 102 teenagers with NPC received external irradiation, among them, 6 cases with N3 diseases was treaed with chemotherapy simutaneously. The tumor dose of primary site was 38Gy-82Gy with an average dose of 66Gy. The number of patients with stage Ⅲ-Ⅳ was 86 which instituted 85% of the whole groupe. Reaults: After a minimum follow-up of 5 years, the overall 5 years survival rate was 58.6%. Administration of RT +CT to 6 cases with N3 disease survived 3 cases. The main cause of deaths was remote metastases. Sterility and secondary amenorrhea was found in this group. Conclusion: (1) Applications of chemotherpy may improve the suvives of patients with N3 disease. (2) The protection of hypophysis of the youngs against irradiation should be emphasized.
