Advanced Search
CHEN Yu-qiang, CHEN Zheng-ming, WU Qiao, SU Wen-jin. Effects of Retinoic Acid on Gastric Cancer Cells' Differentiation and Relevant Enzmye Activity[J]. Cancer Research on Prevention and Treatment, 1999, 26(4): 252-254.
Citation: CHEN Yu-qiang, CHEN Zheng-ming, WU Qiao, SU Wen-jin. Effects of Retinoic Acid on Gastric Cancer Cells' Differentiation and Relevant Enzmye Activity[J]. Cancer Research on Prevention and Treatment, 1999, 26(4): 252-254.
shu

Effects of Retinoic Acid on Gastric Cancer Cells' Differentiation and Relevant Enzmye Activity

  • The State Lab.for Tumor Cell Engineering of Xiamen University, Xiamen 361005

More Information
    Abstract
  • Objective: To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity. Methods: Treated with 10-6 mol/L all-trans retinoic acid(ATRA), gastric cancer cells, BGC-823 and MKN-45, were measured by flow cytometry for cell cycle distribution, by microplate reader for enzyme activity, by 3H-TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate. Results: After treated with ATRA, BGC-823 was lead to stay in G0/G1 phase, while LDH, ALP and β-G activity was suppressed, the inhibition rates were 22.40 %, 32.94 % and 41.35 %, respectively. DNA synthetic speed was decreased by 41.43 % and growth inhibition rate was 61.03 %. Nevertheless, MKN-45 can't be changed as BGC-823. Conclusion: BGC-823, but not MKN-45, can be induced differentiation and cell growth can be suppressed by ATRA.
    • FullText(HTML)
    • References (13)
  • [1]
    Gudas LJ, etal. Cellular biology and biochemistry of the retinoids. In “The Retinoids”, 2nd ed., 1994, p443-520, Raven Prsdd ,New York;
    [2]
    刘卫, 房殿春.维甲类化合物诱导肿瘤细胞分化研究进展.国外医学内科学分册, 1996 ,23: 160-163;
    [3]
    Kim YS .etal.Effects of sodium buti rate, dimethy sulfoxide and retinoic acid or glycolipids on human renal adenocarcinoma cells. Cancer Res, 1984, 44 :1648-1652;
    [4]
    孙月霞, 等.维甲酸对人胃癌细胞表型的逆转作用.肿瘤, 1994, 14: 85-87;
    [5]
    M .Machlas, etal.The Determination of Lactic Dehydrogenase with a Tetrazolium Salt.Anal.Biochem, 1960, 1: 317-326;
    [6]
    Gracia-Rozas C, etal. Alkaline Phosphatase Activity as a Memberame Marker for Activated B cells. J Immunol, 1982, 129(1): 52-53;
    [7]
    Fichman WH, etal. Human Serum β-Glucuronidase; Its Measurement and Some od Its Properties. Clin Chim Acta, 1967, 15(3): 435-447;
    [8]
    吴乔, 等.ATRA 受体转录水平的改变与癌细胞生长的关系.厦门大学学报(自然科学版), 1997, 36: 787-794;
    [9]
    糜漫天, 等.ATRA 诱导细胞分化分子机制的研究进展.国外医学分子生物学分册, 1998, 20: 17-22;
    [10]
    Mehta K, etal. Activation of retinoid receptors RARαand RXRα induces differentiation and apoptosis, respectively, in HL60 cells.Cell Growth Differ, 1996, 7: 179-186;
    [11]
    Shao Z-M, etal. p53 independent G0/G1 arrest and apoptosis induced by a novel retinoid in human breast cancer cells. Oncogene, 199511: 493-504;
    [12]
    汤钊猷主编.现代肿瘤学.上海:上海医科大学出版社, 1993: 47-70;
    [13]
    Nowak G, etal.Hypoxi a and proliferation are primory responsible for induction of lactate dehydrogenase activity in cultured cells.J Toxi cology &Envi ronmental Health, 1996, 49: 439-452.
    • Related Articles

  • Related Articles

    [1]LI Hong-sheng, YANG Bo, HE Wei-xing, PENG Jun-qin, LU Min-ying, QIAN Peng-fei. Inhibitory Effect of Activated CD40Lig with Docetaxel on Breast Cancer Cell Proliferation[J]. Cancer Research on Prevention and Treatment, 2010, 37(05): 522-524. DOI: 10.3971/j.issn.1000-8578.2010.05.009
    [2]XU Pei-rong, FAN Tian-li, LIU Chong, LU Zhao-ming, LIU Hongtao, GONG Tian-xiao, XUE Le- xun. Activation of Notch1 Signaling Pathway in Esophageal Squamous Cell Carcinoma and Its Effect on Cell Cycle[J]. Cancer Research on Prevention and Treatment, 2009, 36(11): 908-913. DOI: 10.3971/j.issn.1000-8578.2009.11.003
    [3]FEI Ji-min, JIANG Yong-xin, LI Mei, CHEN Yun, YANG Jie. Effect of Hyperthermia on Tongue Squamous Carcinoma Cells Proliferation Activity[J]. Cancer Research on Prevention and Treatment, 2007, 34(12): 901-903. DOI: 10.3971/j.issn.1000-8578.66
    [4]LAN Mei, SHI Yong-quan, HAN Zhe-yi, NING Xiao-xuan, FAN Dai-ming. Effect of Changes in Intracellular Calcium on Hypoxic Inducible Factor-1 Expression and Transcriptional Activity in Human Gastric Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2007, 34(09): 659-662. DOI: 10.3971/j.issn.1000-8578.2528
    [5]ZHAO Ying-hai, CHEN Xiao-yi. Effect of Cisplatin on Telomerase Activity and Cell Cycle and Apoptosis in Human Nasopharyngeal Carcinoma CNE-2Z Cells[J]. Cancer Research on Prevention and Treatment, 2006, 33(05): 305-307. DOI: 10.3971/j.issn.1000-8578.3367
    [6]CUI Yu lan, NI Jiang, LIU Guo yi. Effect of Estrogen and Progestin on Telomerase Activity and Cell Cycle in Endometrial Carcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2004, 31(07): 403-405. DOI: 10.3971/j.issn.1000-8578.272
    [7]JI Xue-mei, ZHOU Yun-feng. The lnduction of Cisplatin (CDDP)and Radiation on Telomerase activity of Human Laryngeal Neoplasms Cells and Explore the Mechanism[J]. Cancer Research on Prevention and Treatment, 2001, 28(04): 289-291. DOI: 10.3971/j.issn.1000-8578.1767
    [8]Huang Dinan, . The effect of quercetin on RNA polymerases and cell cycle in human leukemia HL-60 cell[J]. Cancer Research on Prevention and Treatment, 1998, 25(5): 339-342.
    [9]Leng Jun, . The changes of cell cycle during the mucoepidermoid carcinoma cell apoptosis by Doxorubicin[J]. Cancer Research on Prevention and Treatment, 1998, 25(2): 92-94.

Catalog

    SU Wen-jin

    • The State Lab.for Tumor Cell Engineering of Xiamen University, Xiamen 361005

    1. On this Site
    2. On Google Scholar
    3. On PubMed
    Get Citation
    PDF
    XML
    Article views (1734) PDF downloads (399) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Download Center

    Links

    Contact Us

    Address：116 Zhuodaoquan South Road,
    Hongshan District, Wuhan（430079）

    Tel：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    Email Alert RSS
    Total visits：24642222 Visits today：27430

    Copyright © Editorial Department of Cancer Prevention Research 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return