白血病细胞DNA及CD44的临床研究
The Clinical Study of Leukemia Cell DNA And CD44
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摘要: 通过综合研究不同病期急性白血病、慢粒急变及骨髓增生异常综合征─-难治性贫血伴幼稚细胞增多（MDS－RAEB）患者外周血白血病细胞DNA、细胞周期及CD44的变化，发现其与临床的关系，以指导临床诊断及治疗。应用流式细胞仪（FCM）研究了46例急性白血病，10例慢粒急变及5例MDS－RAEB患者DNA指数、DNA倍体和S期百分率（SP）的分布及血液CD44表达情况，并与正常对照进行了比较。急性白血病、慢粒急变及MDS-RAEB三组患者DNA异倍体检出率相似；急性白血病难治/复发组DNA非整倍体率均高于完全缓解组，且FCM可发现体内存在的低于光镜检测水平的残留白血病细胞。初诊时呈现DNA整倍体及低DNA指数的急性白血病患者具有较好的治疗效果。患者组血液CD44表达与正常对照组间无显著性差异。急性白血病患者制定化疗方案时，DNA指数及DNA非整倍体率可作为一重要参考指标。Abstract: Objective:We studied the peripheral blood leukemic cell DNA, cell cycle and CD44changes of acute leukemia. blast crisis of CML and MDS--RAEB Patients to find their relationship with clinical feature in order to guide the clinical diagnosis and treatment. Methods :By using FCM, the DNA index. DNA ploidy and SPof 46 acute leukemia.10blast crisis of CML.5 MDS--RAEB patients and also blood CD44 expressions were studied in the above patients. Results: There was difference of DNA aneuploidy in acute leukemia.blast crisis of CML and MDS--RAEB Patients i DNA aneuploidy was higher in radical/relapsed acute leukemia patients than that of CR acute leukemia patients. The treatment of acute leukmia patients with diploids and lower DNA index was more effective. There was no difference of blood CD44 expression between patients and urinal controls. Conclusion =DNA index and aneuploidy may be regarded as an important reference in giving chemotherapy regimens of acate leukemia.
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