miR-206通过调节ERα水平影响乳腺癌细胞MCF-7对他莫昔芬敏感度的初步研究

doi:10.3971/j.issn.1000-8578.2015.11.003

miR-206通过调节ERα水平影响乳腺癌细胞MCF-7对他莫昔芬敏感度的初步研究

Influences of miR-206 on Sensitivity of Estrogen Receptor-α Positive Breast Cancer Cells MCF-7 to Tamoxifen

摘要

摘要: 目的探讨乳腺癌细胞株MCF-7中miR-206能否调节ERα表达水平并影响乳腺癌细胞对他莫昔芬的敏感度。方法将miR-206 mimic、miR-206 inhibitor及相应阴性对照（miR-206 mimic NC、miR-206 inhibitor NC）分别转染乳腺癌MCF-7细胞，RT-PCR法检测转染后各组乳腺癌细胞中miR-206和ERα mRNA的表达量，用5 μg/ml他莫昔芬处理MCF-7细胞后，MTT法检测不同转染组细胞增殖情况。结果 miR-206 mimic组中乳腺癌细胞miR-206表达升高，ERα mRNA表达降低；miR-206 inhibitor组中乳腺癌细胞miR-206表达降低，ERα mRNA表达升高。他莫昔芬作用24 h后，miR-206 mimic组与阴性对照组（miR-206 mimic NC）的乳腺癌细胞增殖抑制差异无统计学意义（t=-1.169, P=0.276）；miR-206 inhibitor组的乳腺癌细胞增殖抑制明显强于阴性对照组（miR-206 inhibitor NC）（t=-3.054, P=0.016）。结论下调miR-206表达可以增加ERα阳性乳腺癌细胞对他莫昔芬的敏感度。

Abstract: Objective To investigate the influences of miR-206 on estrogen receptor-α expression and the sensitivity of breast cancer cells MCF-7 to tamoxifen. Methods The levels of miR-206 and ERα mRNA of breast cancer cells MCF-7 were quantitated by RT-PCR; the change of expression of miR-206 and ERα mRNA were quantitated after MCF-7 cells were transfected by miR-206 mimic, inhibitor and corresponding negative controls for 24h; cell growth was measured by MTT after transfected MCF-7 cells were treated with 5ug/ml tamoxifen for 24h. Results Compared with corresponding negative controls, miR-206 mimic decreased ERα mRNA expression while increased miR-206 expression in MCF-7 cells but had no notably effect on cell growth after tamoxifen treatment for 24h(t=-1.169, P=0.276 ); miR-206 inhibitor increased ERα mRNA expression while decreased miR-206 expression in MCF-7 cells and had notably effect on cell growth after tamoxifen treatment for 24h (t=-3.054, P=0.016). Conclusion Downregulating miR-206 expression could increase the sensitivity of ERα positive breast cancer cells MCF-7 to tamoxifen.

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