扁柏醇联合氯喹对人肺腺癌细胞凋亡的影响

doi:10.3971/j.issn.1000-8578.2015.10.004

扁柏醇联合氯喹对人肺腺癌细胞凋亡的影响

Effect of Hinokitiol Combined with Chloroquine on Apoptosis of Human Lung Adenocarcinoma Cells

摘要

摘要: 目的探讨扁柏醇（Hinokitiol）联合氯喹对人肺腺癌（H1975）细胞凋亡的影响。方法选用人肺腺癌H1975细胞系，应用MTT法检测扁柏醇和（或）氯喹对H1975细胞活性的影响，Westernblot法检测扁柏醇和（或）氯喹对H1975细胞凋亡通路相关蛋白（Cleaved PARP、Cleaved Caspase-3、Bax）表达水平的影响。结果 0、2.5、5、10 μmol/L扁柏醇作用H1975细胞24 h后，呈剂量依赖性地抑制H1975细胞增殖，H1975细胞凋亡相关蛋白（Cleaved PARP、Cleaved Caspase-3、Bax）表达水平升高。当氯喹和扁柏醇联合应用时，与单用扁柏醇相比，显著降低H1975细胞存活率，并提高H1975细胞凋亡率，凋亡相关蛋白Cleaved PARP、Cleaved Caspase-3表达水平显著增加。结论氯喹增加扁柏醇诱导人肺腺癌H1975细胞凋亡。

Abstract: Objective To investigate the effect of Hinokitiol combined with chloroquine on the apoptosis of human lung adenocarcinoma cell line H1975. Methods Human lung adenocarcinoma cell line H1975 was chosen. The viability of H1975 cells treated with Hinokitiol and(or) chloroquine were determined by MTT. The expression of apoptosis-related proteins, Cleaved PARP, Cleaved Caspase-3 and Bax, were detected by Western blot. Results After treated with 2.5, 5, 10μmol/L Hinokitiol for 24h, the viability of H1975 cells was significantly decreased in a dose-dependent manner, moreover, the apoptosis-related proteins, Cleaved PARP, Cleaved Caspase-3 and Bax, were upregulated by hinokitiol. The viability of H1975 cells treated with hinokitiol and chloroquine was significantly decreased, in addition, the apoptosis-related proteins, Cleaved PAR and Caspase-3, were increased, compared with Hinokitiol alone group. Conclusion Chloroquine increased Hinokitiol-induced apoptosis of H1975 cells.

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