Abstract: Objective To study whether patients benefit more from the 12-point method than the 6-point method in prostate biopsy, and recommend suitable point method for treatment. Methods The first time transrectal ultrasound-guided prostate biopsy was performed in 425 cases, among which 224 cases underwent the 6-point puncture method and the other 201 cases underwent the 12-point puncture method. In this study we compared the positive rate of different methods at various PSA levels and the variation of Gleason score before and after surgery. Results In patients with PSA>20ng/ml, the positive rate of 6- and 12-point method were 85.3% and 77.5% (P>0.05) respectively. In patients with 10ng/ml＜PSA≤20 ng/ml, the positive rate of 6- and 12-point method were 33.8% and 39.1% (P>0.05). The 12-point method didn't bring a higher positive rate than the 6-point method. In the group of PSA≤10 ng/ml, the positive rate of 6- and 12-point method was 24.1% and 45.8%, respectively (P<0.05). The 12-point method brought a statistically higher positive rate than the 6-point method. Conclusion For patients with PSA>20 ng/ml, 6-point method is suffi cient for diagnosis and treatment guidance. For patients with PSA≤10 ng/ml, 12-point method is recommended. For patients with 10≤PSA<20 ng/ml, both 6- and 12-point methods are applicable.