人小细胞肺癌P53基因突变的研究
The Study of P53 Gene Mutation in Human Small Cell Lung Cancer
-
摘要: 为探讨小细胞肺癌（SCLC）发生的分子机制，应用免疫组化和聚合酶链反应─-单链构象多态性（PCR－SSCP）技术，对14例SCLC的石蜡切片标本进行了P53基因突变研究。结果显示：P53蛋白免疫组织化学反应有9例出现阳性，其中2例为十，7例为～，阳性率为64.3%（9/14）。P53基因第5、6、7、8外显子突变率分别为21.4%（3/14）；14，3%（2/14）;14.3%（2/14）；7.1%（1/14），总突变率为57.1%（8/14）。提示：SCLC中存在较高的p53基因突变率，P53基因突变在人类肺癌的发生发展过程中起着重要的作用。
-
关键词:
- 小细胞肺癌 /
- 聚合酶链反应──单链构象多态性 /
- P53基因 /
- 点突变
Abstract: To discuss the molecuar mechanism of the small cell lung cancer (SCLC). P53 gene mutation in SCLC of 14 paraffin embedded tissue section was observed by immunohistochemical staining (LSAB method) and polymerase chain reaction and single-strand conformation polymorsphism (pcr-SSCP) analysis. The results showed that the positive rate of immunohistochemical reaction was 64. 3% (9/14). of which 2 cases are, 7 cases are ～ 。 P53 gene point mutation in exon 5, 6, 7 and 8 are respectively: 21. 4% (3/14) ；14. 3% (2/14)；14. 3% (2/14) ；7. 1 % (1/14). The total mutation rate is 57. 1 % (8/14). It was suggested that Comparatively high mutation rate of P53 gene exists in SCLC,which might be the crucial gene of cause of the human lung carcinoma. -
-
1 Lim Ay thed DC Recen developments in trement of lung cancer.JAMA.1992,267:1661 2 刘彤华，诊断病理学，北京：人民卫生出版社。1994：183 3 Spagnole DV,Turbeu GR,Dix B,et al,Polymearse chain reaction and single-strand conformation polymorphism analysis (PCR-SSCP)，a novel means of detecting DNA mutation. Adv Anat Pathol, 1994, 1:61 4 Rygerg G, Kure, E, Lystad S, et al. PS' mutation in lung tumor relationship putative suceptibility markers for cancer. Cancer Res, 1994,54:1551 5 Kishimoto Y, Murakami Y, Shiraishi M, et al. Aberration of the P53 tumor suppressor gene in human non-small cell lung carcinomas of the lung cancer. Cancer Res. 1992, 52:4799 6 Fontanin G, Bigini D, Vignati S. et al. P53 expression in NSCLC: clinical and biological correlations. Anticalcer Res, 1993, 13:737 7 Quinlan DC, Davidson AG, Summres CL, et al. Accumulation of PS' protein correlates with a poor prognosis in human lung cancer. Cancer Res, 1992,52:4828 8 Gaaman J, Ruggen B, MomikiS, et al. Detection of PS' in primary lung tumors and non-small cell lung carvinoma cell line. Am J Pathol, 1991，139:839 9 Hall Pa, Lane Dp. P53 in tumor pathology: can we trust immunohistochemistry7 I pathol, 1994, 172:1 10 Greenbatt MS, Bennett Wp, Hollatein M, et al. Mutation in the P53 tumor suppressor gene clues to cancer etiolgy and molecular pathogenesis. Gancer Res, 1994. 54: 4855
计量
- 文章访问数: 1345
- HTML全文浏览量: 68
- PDF下载量: 273
下载:
