冬凌草甲素对肿瘤细胞钠泵活性的影响

吴孔明; 张覃沐; 王庆端; 华海婴; 齐建英; 张予

冬凌草甲素对肿瘤细胞钠泵活性的影响

河南医科大学

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出版历程
- 刊出日期: 1994-08-04

Effect of Oridonin on the activity of tumor cell sodium pump

Wu Kongming,

Henan Medical University, 450052

摘要

摘要: 以⁸⁶Rb示踪法，观察3种肿瘤细胞的钠系转运活性及冬凌草甲素（Orid.）对其的影响。结果表明：（Orid.）腹腔注射及体外作用均能显著抑制艾氏腹水瘤细胞的钠系转运功能；体外作用12小时，半数抑制浓度约为5ug/ml，在此浓度下，药物作用1小时的抑制率为22%。同时测定人癌细胞系7901，Hep-2的钠泵活性高于人胚肺纤维母细胞系SL，且对Orid.较敏感。本文结果提示Orid.抗癌机理可能与钠泵活性抑制有关，从生物膜转运角度进一步证明Orid.的抗肿瘤作用。
- 冬凌草甲素 /
- 肿瘤细胞 /
- 钠泵
Abstract: The sodium pump activity of tumor cells and effect of Oridonin (Grid.) were measured by using ⁸⁶Rb trancing method. The results showed that serum stimulus increased pump activity of EAC cell in vitro;while Orid inhibited the ⁸⁶Rb infulx of EAC cell in vitro (1-7.5ug/ml) and in vivo (5-20mg/(kg·d) × 3). preincubating cell with Orid (5ug/ml) for 1, 3, 6, 9h, the inhibitory rates were 22%, 39%, 50%, 63%, respectively. The pump activity of tumor cell lines 7901, Hep-2 and normal cell line SL were 4255, 4681 and 2632; the inhibitiry rates of Orid. (5ug/ml) were 42%, 51% and 20% separately. These data suggested that Orid. can restrain tumor cell growth by means of inhibiting sodium pump activity.
- Oridonin /
- tumor /
- ⁸⁶Rb influx

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参考文献(5)

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