Study on the role of P53 gene transfer on human glioma cell growth in nude mice
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摘要: 目的 :探讨转染野生型P53(wt-P53)和突变型P53(mtP53)基因,对人胶质瘤细胞株SHG44裸鼠致瘤性的影响及意义. 方法 :采用质脂体介导法,分别将wt-P53和mt-P53基因导入人胶质瘤细胞株SHG44,体内外检测转导细胞的生长状况和裸鼠致瘤性. 结果 :转染mt-P53基因的细胞株,G418筛选细胞集落数、软琼脂平皿细胞集落数以及裸鼠瘤组织重量和体积,均显著高于对照组(P <0.01);而转染Wt-P53 基因的细胞株均显著低于对照组(P<0.01),表明导入wt-P53基因的细胞株,瘤细胞生长速度明显低于对照细胞株和导入.mt-P53基因的细胞株,即导入mt-P53基因的细胞株瘤细胞生长速度最快,而导入Wt-P53基因的细胞株瘤细胞生长速度最慢. 结论 :Wt-P53基因能有效地抑制人胶质瘤细胞生长;mt-P53基因则可以明显地促进瘤细胞生长.Abstract: Objective To explore the significance and role of P53gene transfer on human glioma cell growth in nude mice. Method Wild-type P53 gene(wt-P53)and mutant P53 gene(mt-P53)were transfected into the human glioma cell line SHG44.And the growth of gene-transfected cell lines were observed in vitro and in vivo. Results The tumor resulting from the cells transfected with the wt-P53 gene more slowly and was smaller than that from control SHG44 cells.In contrast,the tumor from the ce-lls transfected with the mt-P53 gene grew faster than that produced by cells transfected with the Wt-P53 gene and that produced by control SHG44 cells. Conclusion The wild-type P53 gene could inhibit the glioma cell growth in nude mice and the mutant P53 gene could enhance the glioma cell growth in nude face.
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Key words:
- Anti-oncogene /
- P53 gene /
- Glioma /
- Tumorigenicity in nude
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