S180瘤苗和BCG抗癌作用及诱发肿瘤细胞凋亡
Antitumor Activity of Tumor cell vaccine with BCG and Its Induction of ApoPtosis inTumor Cells
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摘要: 用S180瘤苗(可溶性抗原)及BCG皮内注射治疗荷瘤小鼠, 显示肿瘤生长受抑, 脾脏CTL活性增强, 与对照组或单一治疗组(瘤苗组、BCG组)相比P<0.01, 而荷瘤前6天治疗更显著。 治疗组的瘤组织作超微形态观察, 有核质凝聚胞浆固缩等细胞凋亡的改变，作流式细胞术DNA直方图显示在G1期前具凋亡特征的AP峰较对照明显升高P<0.01。 实验显示, 瘤苗BCG可能通过CTL启动细胞凋亡途径抑制肿瘤生长。Abstract: The tumor bearing mice were injected id with S180 tumor cellvaccine (soluble tumor antigens) and BCG. The tumor growth was inhibited and the cytotoxicity of CTLwas increased. Both showed significant (P<0.01 ) difference than that of control groupor alone treatment group (tumor cell vaccine or BCG). The angment was much more significant when treatment was used in 6 days previously. Ultrastructure and floW cytometry showed in treatment group tumor tissue cell, the morphological change of apoptosisare comprises nuclear and cytoplasmic condensation. Content frequency histograms all cycle analysis revealed that the feature of apoptoic cell were observed in treatment group P<0.01. The results suggested the inducement of an apoptotic proceed in murine S180tumor cells which were treated by tumor cell vaccine with BCG during tumor regression.
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Key words:
- Tumor cell vaccine /
- BCG /
- CTL cell /
- Apoptosis
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[1] Mitchell MS, Kan-Mitchell J, Kempf RA, et al. Active specific immunotherapy for melanoma:phasel trial of allo-genic lysates and a Novel adjuvant. Cancer Res, 1988, 48:5388. [2] Hanna MG. Ransom JH, Peters L, et al. Active specific im-muno therapy of human coloreactal carcinoma with an au-tologous tumor cell/BCG. New York Acadamy of Science, 1993, 12:135. [3] 张元杏, 李振荣, 张艳林等复方何首乌对IL-2、LAK和巨噬细胞抗癌活性的增强作用, 中国癌症研究进展. 云南科技出版社, 19944:188. [4] Kerr JER, Winterford CM, Appl AD, et al. Apoptosis:Its significance in cancer and cancertherapy. Cancer, 1944, 73:2013. [5] Darzynkiewicz Z, Bruno S, Bino GP, et al. Features of apoptotic cells measure by flow cytometry. Cytometry, 1992, 13:795. [6] Chang AE, Yoshizawa H, Sakai K, et al. Clinical observa-tion on adoptive immunotherapy with vaccineprimed T-lymphocytes Secon-darily sensitized to tumor in vitro. Cancer Res, 1933, 53:1043. [7] Heusel JN, Wesselschmidt Rl, Shresta Set al. Cytotoxici lymphocyteds require Granzyme B for the rapid induction of DNA fragmenation and apoptosis in allogeneic target cells. Cell, 1994, 76:977. [8] Urbab JL, Shepard HM, Rothstoin JL, et al. Tumor necro-sis factor:A Potent effector molccule for tumor cell killing by activated macrophages. Proc Natl Acad Sci USA, 1986, 83:5233.
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