SRRS方剂抑制消化道恶性肿瘤P53基因突变的实验研究
The Effect of SRRS Prenventing P 53 Gene from Mutation
-
摘要: 目的：从分子水平探讨SRRS方剂治疗消化道癌的可能机制。方法：选用Lovo结肠腺癌细胞裸小鼠移植瘤模型，观察中药SRRS方剂抑制移植瘤生长的作用，并用PCR-SSCP银染色法研究SRRS方剂对Lovo移植瘤生长过程中P53基因突变的影响情况。结果：对照组有50%(5/10)发生P53基因第七外显子突变，SRRS方剂治疗组无(0/10)此现象(P<0.05)。结论：提示SSRS方剂能抑制lovo移植瘤生长过程中P53基因的突变，即SRRS方剂可对肿瘤生长过程中肿瘤细胞的基因水平有影响作用。Abstract: In order to study the mechanism of the tumor suppressor effect of the “SRRS”,a recipe composed of some Chinese herbs,the mutation of P 53 gene exon 7 in the tumor tissue was observed by PCR SSCP in the nude mice Lovo transplantation tumors.The important observation of this study was that none of the samples(0/10)was shown to have P 53 gene exon7 mutation in the experimental group which SRRS was taken.After all,50%(5/10) of the samples were shown to have P 53 mutation in the control group.This result suggested that SRRS could prevent P 53 gene from mutating during the Lovo transplatstion tumors growing.
-
Key words:
- SRRS /
- P53 /
- suppressor /
- PCR-SSCP
-
-
1 邱佳信，等.晚期恶性肿瘤的异病同治——健脾法在消化道恶性肿瘤中的应用.上海中医药杂志，1984，1：2～4 2 邱佳信，等.健脾法为主治疗晚期胃癌的探讨.中医杂志，1992，8：23 3 邱佳信，等.中医中药对肿瘤预防作用的探讨.中医杂志，1993，9：560～561 4 Sambrook J,et al.Molecular cloning.Cold Spring Harbor Laborator Press,1989,954～961 5 Fearon ER,Vegelestein B. A genetic model for colorectal tumor genesis.Cell,1990,61:759～767 6 Levine AJ,et al.The P53 tumar suppressor gene.Nature,1991,351:453 7 Greenblatt MS,et al.Mutattions in the P53 tumor suppressor gene:clues to cancer etiology and molecular pathoghenesis.Cancer Res,1994,54:1855～1878 8 Sugano K,et al.Methods in laboratory investigation rapid and simple detection of c-ki-ras 2 gene codon 12 mutations by nonradioisotopic single-strand conformation polymorphismn analysis.Laboratory Investigation,1993,68:361～366 9 Pocard M,et al.Different P53 mutations produce distinct effects on the ability of colon carcinoma cells to become locked at the G1/S boundary after irradiation.Oncogene,1996,12(4):875～882 10 邱佳信，等.健脾补肾中药对肿瘤成因多阶段学说中起始和启动的影响.中国医药学报，1993，8(5)：16～19
计量
- 文章访问数: 1472
- HTML全文浏览量: 65
- PDF下载量: 479
下载:
