Effect of SABP, A Water-soluble Component of Salvia Miltiorrhiza, on Immune Microenvironment of Mice with Liver Cancer
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摘要:目的
探讨丹参水溶性组分SABP影响H22细胞肝癌原位移植瘤生长及肝癌免疫微环境的效应。
方法建立H22细胞肝癌原位移植瘤小鼠模型。ELISA检测肝脏中PD-L1、TGF-β、IL-1β、IL-10、IL-4、IFN-γ、IL-18、IL-7、IL-2、CCL-2和CCL-21的表达量。计算肝脏、脾脏和肾脏的脏器系数。
结果SABP抑制H22细胞肝癌原位移植瘤的生长，提高肝癌微环境中PD-L1、TGF-β、IL-1β和IL-10的表达水平，增加了肝、脾和肾系数。
结论SABP能抑制H22细胞肝癌原位移植瘤生长，促进肝癌微环境中PD-L1、TGF-β、IL-1β和IL-10抑制性免疫分子的表达。
Abstract:ObjectiveTo investigate the effect of SABP, a water-soluble component of Salvia miltiorrhiza, on the growth of orthotopic transplantation of H22 liver cancer and the immune microenvironment of liver cancer.
MethodsWe established a mouse model of orthotopic transplantation of H22 cell liver cancer in BALB/c mice. ELISA was used to detect the expression of PD-L1, TGF-β, IL-1β, IL-10, IL-4, IFN-γ, IL-18, IL-7, IL-2, CCL-2 and CCL-21 in the liver. We counted the organ indexes of liver, spleen and kidney.
ResultsSABP inhibited the growth of orthotopic transplantation tumors of H22 cell liver cancer, and increased the expression levels of PD-L1, TGF-β, IL-1β and IL-10 in the microenvironment of liver cancer, as well as the liver, spleen and kidney coefficients.
ConclusionSABP could inhibit the growth of orthotopic transplantation tumors of H22 cell liver cancer and promote the expression of PD-L1, TGF-β, IL-1β and IL-10 in the microenvironment of liver cancer.
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Competing interests: The authors declare that they have no competing interests.作者贡献：李圣豪：实验实施，论文撰写郝立园、国英琳：数据分析，论文修订彭晴、丁静茹：数据收集、整理及分析石新丽：实验设计，论文审核
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图 1 SABP抑制H22细胞肝癌原位移植瘤的生长
Figure 1 SABP inhibited growth of orthotopic transplantation of H22 cell liver cancer
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图 2 SABP对H22细胞肝癌原位移植瘤小鼠体重(A)、脾重(B)和肝、脾、肾系数(C~E)的影响
Figure 2 Effect of SABP on body weight(A), spleen weight(B) and coefficients of liver, spleen and renal(C-E) of orthotopic transplantation of H22 cell liver cancer in mice
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