Effects of Cellular Immunity,IL-2 and TNF-α from Opioid Drugs on Hepatic Cancer Mice
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摘要: 目的 探讨不同的阿片类药物对肝癌小鼠细胞免疫功能及IL-2和TNF-α的影响。方法采用细胞接种的方法建立H22肝癌小鼠动物模型，随机分成5组，每组10只，流式细胞仪技术检测肝癌小鼠T淋巴细胞亚群中CD3+、CD4+、CD8+、CD4+/CD8+ 数值,双抗体夹心法（ELISA）检测小鼠血清IL-2和TNF-α数值。结果与0.9%氯化钠溶液组相比哌替啶组中肝癌小鼠T淋巴细胞亚群中CD3+、CD4+、CD8+、CD4+/CD8+没有明显变化（P>0.05），吗啡组、芬太尼组和舒芬太尼组均明显降低（P<0.05）；与0.9%氯化钠溶液组比较哌替啶组小鼠血清IL-2含量明显升高（P<0.05），血清TNF-α含量无明显差别（P>0.05），与0.9%氯化钠溶液组比较吗啡组、芬太尼组、舒芬太尼组血清IL-2和TNF-α均明显降低(P<0.05)。 结论吗啡、芬太尼、舒芬太尼对肝癌小鼠T淋巴细胞亚群和IL-2、TNF-α明显抑制。哌替啶对肝癌小鼠T淋巴细胞亚群抑制不明显，能提高血中IL-2含量，对TNF-α水平无明显抑制。
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关键词:
- 阿片类药 /
- 肝癌 /
- 细胞免疫功能 /
- IL-2 /
- TNF-&alpha
Abstract: Objective To study the effects of cellular immunity，IL-2 and TNF-α caused by Opioid drugs on hepatic cancer mice. Methods The models of H22 hepatic cancer mice were established by the cell inoculation method;and the animals were randomly divided into 5 groups.10 rats in each group were picked up randomly to detect of lymphocyte subsets CD3+,CD4+,CD8+and CD4+/CD8+ counts.The number of IL-2 and TNF-α were detected by Enzyme-linked Immunosorbent Assay(ELISA). Results Compared with the group Saline，the numbers of CD3+,CD4+,CD8+ and the ratios of CD4+/CD8+showed no significant different in group Pethidine（P>0.05），but decreased significantly in group Morphine，group Fentanyl and group Sufentanil（P<0.05）.Compared with group Saline，the numbers of IL-2 increased significantly in group Pethidine（P<0.05）;and the numbers of TNF-α showed no significant difference（P>0.05）;but the numbers of IL-2 and TNF-αdecreased significantly in group Morphine，group Fentanyl and group Sufentanil （P<0.05）. Conclusion Morphine,Fentanyl and Sufentanil can suppress the immune function，significantly decrease IL-2 and TNF-α of hepatic cancer in mice.Pethidine suppress the immune function unobviously，and the numbers of TNF-α has no significant difference，but significantly increase the numbers of IL-2.-
Key words:
- Opioid drugs /
- Hepatic cancer /
- Cellular immune function /
- IL-2 /
- TNF-&alpha
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[1] Tsai YC，Won SJ，Lin MT.Effects of morphine on immune response in rats with sciatic constriction injury[J].Pain，2000，88(2)：155-60. [2] Bar-Yosef S，Melamed R，Page GG，et al.Attenuation of the tumor-promoting effect of surgery by spinal blockade in rats[J].Anesthesiology，2001，94(6)：1066-73. [3] Zhang Y，Xu HW，Zhang TP，et al.Establishment and improvement of primary hepatocarcinoma ICR mouse model by injection method [J].Xi Bao Sheng Wu Xue Za Zhi，2007，29(3)：434-8.[张煜，徐涵文，张太平，等.注射法制作ICR小鼠 肝癌模型及其改进[J].细胞生物学杂志，2007，29(3)：434-8.] [4] Lu WF.Medical immunology [M].Beijing：People ′s Health Publishing House，2002:94-6.[陆慰蜂.医学免疫学[M] .北京：人民卫生出版社，2002，94-6.] [5] Gao P，Zhou AM.Malignancies in patients with red blood cell immunity and cytokines,and the relationship between free radicals [J].Di Si Jun Yi Da Xue Xue Bao，2005，26(10)：908-11.[高萍，邹爱民.恶性肿瘤患者红细胞免疫 和细胞因子与自由基关系的研究[J].第四军医大学学报，2005，26(10)：908-11.] [6] Xia H，Li J，Yu CH，et al.Effect of TNF-α on the apoptosis of A549 cells[J].Zhongguo Lin Chuang Yao Li Xue Yu Zhi Liao Xue，2005，10(12)：66-8.[夏晖，李捷，于长海，等.TNF-α对人肺癌A549 细胞凋亡的影响[J].中国临床药理学 与治疗学，2005，10(12)：66-8.] [7] Li W，Tang HZ，Jiang YB，et al.Influence of Different Doses of Fentanyl on T- lymphocyte Subpopulations and Natural Killer Cells of Patients with Esophageal Tumor during Preoperation and Postoperation[J].Ai Zheng，2003， 22(6)：634-6.[李伟，汤惠珍，蒋艳波，等.不同剂量芬太尼对食管癌根治术患者术前术后T淋巴细胞亚群、NK细胞的影响[J].癌 症，2003，22(6)：634-6.] [8] Sacerdote P.Opioid-induced immunosuppression[J].Curr Opin Support Palliat Care，2008，2(1)：14-8. [9] Sasamura T,Nakamura S,Iida Y,et al.Morphine analgesia suppresses tumor growth and metastasis in a mouse model of cancer pain produced by orthotopic tumor inoculation[J].Eur J Pharmacol,2002,441(3)：185-91. [10] Cheng WF，Chen LK，Chen CA，et al.Chimeric DNA vaccine reverses morphine - induced immunos uppression and tumorigenesis[J].Mol Ther，2006，13(1)：203-10. [11] Hatsukari I，Hitosugi N，Ohno R，et al.Induction of apoptosis by morphine in human tumor cell lines in vitro[J].Anticancer Res，2007，27(2)：857-64. [12] Zhang H，Yang Y，Liu J，et al.Suppressing effect of anesthetic on growth of emt6 breast cancer in mice[J].Ke Ji Dao Bao，2008，26(4)：66-71.[张辉，杨阳，刘静，等.注射麻醉剂抑制小鼠EMT6乳腺肿瘤的生长[J].科技导报， 2008，26(4)：66-71.] [13] Xing CY,Wu MY,Fan HP.Effects of different anesthetic and analgesic protocols on cellular immune function and stress hormone level in patients undergoing lobectomy for esophagus cancer[J].Nanfang Yi Ke Da Xue Xue Bao，2010 ，30(2)：284.[邢翠燕，吴明毅，范海鹏.不同麻醉和镇痛方法对食管癌手术患者细胞免疫功能及应激激素水平的影响[J].南方 医科大学报，2010，30(2)：284.]
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