乳腺癌中cyclinD2 、CDK4 的表达及其临床意义

doi:10.3971/j.issn.1000-8578.3295

乳腺癌中cyclinD2 、CDK4 的表达及其临床意义

Clinical Significance and Expression of cyclin D2, CDK4 in Human Breast Carcinoma

摘要

摘要: 目的研究乳腺癌组织中细胞周期蛋白D2（cyclinD2）、细胞周期蛋白依赖性激酶4（cyclin-dependentkinase4，CDK4）的表达，探讨它们与p27^kip1蛋白表达、与临床病理指标的关系及其预后意义。方法应用免疫组化SP法，检测96例乳腺癌、18例癌旁正常乳腺组织石蜡切片中cyclinD2、CDK4的表达，比较它们与p27^kip1蛋白表达、与临床病理学指标之间的关系。结果 ①96例乳腺癌组织中cyclinD2、CDK4和p27^kip1表达率分别为41.7％、54．2％和38．5％，与正常乳腺组织相比有显著性差异（P〈0．001）。②cyclinD2、CDK4的表达与乳腺癌的组织学分级，核分裂数有关（P〈0．001），和局部复发有关（P〈0．05），而与患者年龄、肿瘤大小、组织类型无关（P〉0．05），CDK4的表达与乳腺癌的淋巴结转移，雌激素受体状态也有密切关系（P〈0．01）。③p27^kip1的表达缺失，与肿瘤大小、组织学分级，核分裂数，有无淋巴结转移以及局部复发均有关系（P〈0．05），而且p27^kip1的表达与eyelinD2、CDK4的表达呈显著负相关（P〈0．叭）。结论 cyclinD2和CDK4的表达与乳腺癌的发生、发展有关，而p27^kip1的表达缺失和CDK4的异常表达与乳腺癌的侵袭、转移及复发有关，可作为判断乳腺癌生物学行为和预后的重要指标。

Abstract: Objective 　To investigate the expression level of cyclinD2 and CDK4 protein in breast carcinoma, and their relationship to p27^kip1protein and clinicopathological factors as well as the prognostic factors in breast carcinoma. Methods 　The SP immunohistochemical technique was used to detect the expressions of cyclinD2, CDK4 and p27^kip1in 96 cases of breast carcinoma and 18 cases of adjacent nontumor normal breast tissue. The relationship between cyclinD2, CDK4 to p27^kip1protein expression and clinicopathological factors were analyzed. Results 　①The positive expressions of cyclinD2, CDK4 and p27^kip1 protein in 96 cases of breast carcinoma were 41. 7 %, 54. 2 % and 38. 5 % respectively. There was a significant difference between carcinoma and normal breast tissue ( P < 0. 001) . ②The expression level of both cyclinD2, and CDK4 protein in breast carcinoma was significantly associated with histological classification, mitotic index, and local recurrence ( P < 0. 05), and little associated with age, tumor size and histological type ( P > 0. 05) . The expression level of CDK4 in breast carcinoma was related to Lymph node metastases and ER status ( P < 0. 01) . ③The loss of p27^kip1protein expression in breast carcinoma was significantly associated with tumor size, histological classification, mitotic index, Lymph node metastases and local recurrence ( P < 0. 05), There was a negative correlation between p27^kip1 and cyclinD2 as well as CDK4 expression in breast carcinoma ( P < 0. 01) . Conclusion 　The expression of cyclinD2 and CDK4 protein may play a important role in the carcinogenesis and progression of breast carcinoma. The loss p27^kip1expression and over-expressed CDK4 was associated with the invasion, metastases and local recurrence, and may be as the indications of biological behavior and prognosis of breast carcinoma.

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