低频超声对卵巢癌COC1细胞增殖和凋亡的影响
Influence of Low Frequency Ultrasound on Proliferation and Apoptosis of Human Ovarian COC1
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摘要: 目的 观察低频超声对人卵巢癌COC1体外生长的影响。方法 以低频超声 (频率 0 .2 4MHz,剂量 5 .12W /cm2 × 5s)辐照COC1细胞, 采用MTT观察超声对COC1细胞增殖的抑制作用 ;流式细胞仪(FCM)、TUNEL法和光、电镜检测细胞凋亡。结果 超声波能够显著抑制卵巢癌COC1细胞增殖, 辐照后 12h、2 4h、36h和 4 8h, 其MTT光吸收值与对照组相比显著降低 ;FCM检测超声辐照后 6h的COC1细胞凋亡率达 (2 5 .5 7± 4 .6 8) % ;同时TUNEL法观察到棕褐色着染的凋亡细胞 ;相差显微镜及电镜下可见凋亡小体形成等典型形态学改变。结论 低频超声辐照对人卵巢癌COC1细胞体外生长具有明显的抑制作用, 并可诱导其凋亡。Abstract: Objective To investigate the effect of Low-Frequency Ultrasound on proliferation and apoptosis of human ovarian cell line COC1. Methods The growth inhibitory effect of Ultrasound Exposure( 0.24 MHz, 5.12 W /cm 2× 5 s ) on COC1 cell was observed by MTT colormetric assay;Cell apoptosis was analyzed using flow cytometry(FCM);Terminal deoxynucleotidyl transferase-mediated dUTP-digoxin nick end labeling(TUNEL);Phase contrast light microscope and Electron microscope were applied to detect the morphological c...
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