| Citation: |
HU Xiaoshu, WEN Yiyang, YANG Jinhua. Effect of PTENP1 on Proliferation and Apoptosis of Colorectal Cancer Cells and Its Molecular Mechanism[J]. Cancer Research on Prevention and Treatment, 2022, 49(3): 192-196. DOI: 10.3971/j.issn.1000-8578.2022.21.0819
|
To investigate the effect of PTENP1 on the proliferation and apoptosis of colorectal cancer cells and its molecular mechanism.
We selected 107 cases of colorectal cancer and corresponding adjacent tissues as the research objects. The expression level of PTENP1 was analyzed by fluorescence quantitative PCR. Colon cancer HT29 cells with PTENP1 overexpression (PTENP1 group) and empty vector cell line (control group) were established by lentivirus. The cell proliferation and apoptosis were analyzed by CCK8 and flow cytometry. The PTENP1 target gene was analyzed by bioinformatics and double luciferase reporter genes. The expression level of target protein was analyzed by Western blot.
The expression of PTENP1 in colorectal cancer tissues was significantly lower than that in adjacent tissues (P < 0.05). The expression level of PTENP1 in the control group was significantly lower than that in the PTENP1 group (P < 0.05). Compared with the control group, the cell proliferation ability of the PTENP1 group was significantly decreased (P < 0.05), the apoptosis level was significantly increased (P < 0.05). miR-21 was complementary to PTENP1. Compared with the control group, the expression of miR-21 in the PTENP1 group was significantly down-regulated (P < 0.05), and the expression of PTEN protein was significantly up-regulated (P < 0.05).
PTENP1 and miR-21 competitively bind to regulate the expression of PTEN, and then affect the proliferation and apoptosis of colorectal cancer cells.
Competing interests: The authors declare that they have no competing interests.
| [1] |
Fidler MM, Soerjomataram I, Bray F. A global view on cancer incidence and national levels of the human development index[J]. Int J Cancer, 2016, 139(11): 2436-2446. doi: 10.1002/ijc.30382
|
| [2] |
Anastasiadou E, Jacob LS, Slack FJ. Non-coding RNA networks in cancer[J]. Nat Rev Cancer, 2018, 18(1): 5-18. doi: 10.1038/nrc.2017.99
|
| [3] |
Liu L, Liao JZ, He XX, et al. The role of autophagy in hepatocellular carcinoma: friend or foe[J]. Oncotarget, 2017, 8(34): 57707-57722. doi: 10.18632/oncotarget.17202
|
| [4] |
Yndestad S, Austreid E, Skaftnesmo KO, et al. Divergent Activity of the Pseudogene PTENP1 in ER-Positive and Negative Breast Cancer[J]. Mol Cancer Res, 2018, 16(1): 78-89. doi: 10.1158/1541-7786.MCR-17-0207
|
| [5] |
Qian YY, Li K, Liu QY, et al. Long non-coding RNA PTENP1 interacts with miR-193a-3p to suppress cell migration and invasion through the PTEN pathway in hepatocellular carcinoma [J]. Oncotarget, 2017, 8(64): 107859-107869. doi: 10.18632/oncotarget.22305
|
| [6] |
Yu G, Yao W, Gumireddy K, et al. Pseudogene PTENP1 functions as a competing endogenous RNA to suppress clear-cell renal cell carcinoma progression[J]. Mol Cancer Ther, 2014, 13(12): 3086-3097. doi: 10.1158/1535-7163.MCT-14-0245
|
| [7] |
Ou L, Xiang TY, Hao XY, et al. Reduced long non-coding RNA PTENP1 contributed to proliferation and invasion via miR-19b/MTUS1 axis in patients with cervical cancer[J]. Eur Rev Med Pharmacol Sci, 2020, 24(8): 4132-4144.
|
| [8] |
Ning S, Li X. Non-coding RNA resources[J]. Adv Exp Med Biol, 2018, 1094: 1-7.
|
| [9] |
Liu F, Gao H, Li S, et al. Long non-coding RNA ZFAS1 correlates with clinical progression and prognosis in cancer patients[J]. Oncotarget, 2017, 8(37): 61561-61569. doi: 10.18632/oncotarget.18633
|
| [10] |
Pang EJ, Yang R, Fu XB, et al. Overexpression of long non-coding RNA MALAT1 is correlated with clinical progression and unfavorable prognosis in pancreatic cancer[J]. Tumour Biol, 2015, 36(4): 2403-2407. doi: 10.1007/s13277-014-2850-8
|
| [11] |
Zheng R, Du M, Wang X, et al. Exosome-transmitted long non-coding RNA PTENP1 suppresses bladder cancer progression[J]. Mol Cancer, 2018, 17(1): 143. doi: 10.1186/s12943-018-0880-3
|
| [12] |
Gao X, Qin T, Mao J, et al. PTENP1/miR-20a/PTEN axis contributes to breast cancer progression by regulating PTEN via PI3K/AKT pathway[J]. J Exp Clin Cancer Res, 2019, 38(1): 256. doi: 10.1186/s13046-019-1260-6
|
| [13] |
Zhang R, Guo Y, Ma Z, et al. Long non-coding RNA PTENP1 functions as a ceRNA to modulate PTEN level by decoying miR-106b and miR-93 in gastric cancer[J]. Oncotarget, 2017, 8(16): 26079-26089. doi: 10.18632/oncotarget.15317
|
| [14] |
Wang L, Zhang N, Wang Z, et al. Pseudogene PTENP1 Functions as a Competing Endogenous RNA (ceRNA) to Regulate PTEN Expression by Sponging miR-499-5p[J]. Biochemistry(Mosc), 2016, 81(7): 739-747.
|
| [15] |
Squatrito M, Holland EC. DNA damage response and growth factor signaling pathways in gliomagenesis and therapeutic resistance[J]. Cancer Res, 2011, 71(18): 5945-5949. doi: 10.1158/0008-5472.CAN-11-1245
|
| [16] |
Travis G, Haddadi N, Simpson AM, et al. Studying the oncosuppressive functions of PTENP1 as a ceRNA[J]. Methods Mol Biol, 2021, 2324: 165-185.
|
| [17] |
Li X, Dai Y, Xu J. MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway[J]. Mol Vis, 2018, 24: 485-494.
|
| [18] |
Hao XJ, Xu CZ, Wang JT, et al. miR-21 promotes proliferation and inhibits apoptosis of hepatic stellate cells through targeting PTEN/PI3K/AKT pathway[J]. J Recept Signal Transduct Res, 2018, 38(5-6): 455-461. doi: 10.1080/10799893.2019.1585452
|
| [19] |
Zhao MY, Wang LM, Liu J, et al. MiR-21 suppresses anoikis through targeting PDCD4 and PTEN in human esophageal adenocarcinoma[J]. Curr Med Sci, 2018, 38(2): 245-251. doi: 10.1007/s11596-018-1872-7
|
Figures(4) / Tables(1)
This work is licensed under a Creative Commons Attribution 3.0 License.
Copyright © Editorial Department of Cancer Prevention Research 鄂公网安备 42011102005013号 鄂ICP备2022015867号
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: