Expression and Correlation Studies of Id-1, MMP-9, and LMVD in Colorectal Adenocarcinoma Tissues

Objective To explore the expressions of Id-1 and MMP-9 in colorectal carcinoma tissues and their correlation with lymphatic microvessel generation,and their clinical significance.

Methods The expression of Id-1,MMP-9 and LMVD in colorectal adenocarcinoma tissues(50 cases) and colorectal peri-cancer tissues(50 cases) were examined by immunohislochemistry. Using D-240 mRNA as a marker,the LMVD value of colorectal adenocarcinoma tissues was measured.

Results The positive expression of Id-1 and MMP-9 were 72.00% and 78% in colorectal adenocarcinoma tissues,which was significant higher than 24.00% and 28.00% in colorectal peri-cancer tissues(all P＜0.01) . The LMVD value was (15.18±2.16) in colorectal adenocarcinoma tissues markedly higher than (5.24±1.09) in colorectal peri-cancer tissues(P＜0.01) . Expression of Id-1,MMP-9 and LMVD value were correlated with the depth of tumor invasion,TNM stage,lymph node metastasis,vessel invasion,and liver metastasis(all P＜0.01) . The LMVD value in Id-1 positive expression group was significantly higher than in Id-1 negative expression group (P＜0.01) . And the LMVD value in MMP-9 positive expression group was obviously higher than in MMP-9 negative expression group in colorectal adenocarcinoma tissues (P＜0.01) . The expression of Id-1 was obviously correlated with MMP-9 in colorectal adenocarcinoma tissues.

Conclusion  The high expression levels of Id-1 and MMP-9 have a close correlation with the invasion and metastasis of colorectal carcinoma. The up-regulation of them may play an important role in lymphatic microvessel generation and lymph node metastasis in colorectal carcinoma.