EFFECT OF AME ON PROLIFERATIVE RESPONSE IN HUMAN LYMPHOCYTES

In this paper, we investigated the suppressive effect of AME (alternariol monomethyl ether) on proliferative response in human lymphocytes by use of PHA-induced lymphocyte proliferation SH-TdR incorporation method. The results showed: AME had a significant inhibitory effect on SH-TdR incorporation into lymphocytes. As the concentration of AME was 25ug/ml, 50ug/ml, 75ug/ml and 100μg/ml, its suppressive ratio was 76.9%, 89.2%, 91.3% and 94.9% respectively. The effect of AME was significantly different from control (P0.01), and had does-dependent fashion (Y=12.13-0.10X,r=-0.927, P0.05, alone). This result suggests that AME could inhibit cellular immune and affect the AME-induced carcinogenesis.