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Wang Bao-cheng, Guo Jun, Di Jian-shi, Shi Qiu-li, Yao Chang-ying. Establishment of multidrug resistance cell strain of liver cancer and reserch of its multidrug resistance mechanism[J]. Cancer Research on Prevention and Treatment, 1997, 24(5): 263-265.
Citation: Wang Bao-cheng, Guo Jun, Di Jian-shi, Shi Qiu-li, Yao Chang-ying. Establishment of multidrug resistance cell strain of liver cancer and reserch of its multidrug resistance mechanism[J]. Cancer Research on Prevention and Treatment, 1997, 24(5): 263-265.

Establishment of multidrug resistance cell strain of liver cancer and reserch of its multidrug resistance mechanism

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  • For serching establishing human liver cancer multidrug resistance cell strain and reserch its drug resistance mechanism,the BEL-7402 cells were cultured orubicin by progressively cell strain BEL-7402/Dox increase concentration method,and the drug with Dox-resistance were selected for a resistance increased of BEL-7402 cells to vincristinelong period screening cultural.The (VCR) and other 8 anticancer drugs 27-1100 times respectivly by MTT assay.The MDR1 expression product membrane efflux p-glycoprotein and multidrug resistanceassociated(MRP)as well as GSH/GST were examined,the MDR and MRP gene expression were also detected using RT-PCR.The results showed that p-gp level of BEL-7402/Dox were 93.5 to 97.4%MRP were 84.7 to 90.2%,the level of MDR and MRP mRNA expression were also relatively high,no over experssion of GSH/GST was found.
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