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CHEN Dao-zhen, ZHAN Hui-yin, XUE Wen-qun, ZHANG Li-shan, LU Xiao-xuan, FAN Qi-ying, HUANG Xue-wen, WANG Jing, LI Su-feng, SU Ning, LIU Lu, HUANG Ying, TONG Guan-shen. A Study of the RV-HSV-TK/GCV Suicide Gene Therapy System in Breast Carcinoma[J]. Cancer Research on Prevention and Treatment, 2004, 31(12): 725-729. DOI: 10.3971/j.issn.1000-8578.2066
Citation: CHEN Dao-zhen, ZHAN Hui-yin, XUE Wen-qun, ZHANG Li-shan, LU Xiao-xuan, FAN Qi-ying, HUANG Xue-wen, WANG Jing, LI Su-feng, SU Ning, LIU Lu, HUANG Ying, TONG Guan-shen. A Study of the RV-HSV-TK/GCV Suicide Gene Therapy System in Breast Carcinoma[J]. Cancer Research on Prevention and Treatment, 2004, 31(12): 725-729. DOI: 10.3971/j.issn.1000-8578.2066
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A Study of the RV-HSV-TK/GCV Suicide Gene Therapy System in Breast Carcinoma

    Abstract
  • Objective To study the killing effect in vitro and in vivo and the bystander effect of HSV-TK/GCV suicide gene system on mice breast carcinoma cells MA782/5S-8102. Methods GINaTK retroviral vector containing HSV-TK gene was transduced into PA317 packaging cell by lipofectin.Mice breast carcinoma cell line MA782/5S-8102 was infected by high titer viral supernatate. PCR Was resorted to demonstrate the successful transduction of the HSV-TK gene. MA782/5S-8102 /TK cells and MA782/5S-8102 cells were ...
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