Wild-type p53,GM-CSF,B7-1 genes transduced with recombinant adenovirus expression in hepatoma cell lines in vitro

Objective To evaluate the transferring efficiency of recombinant adenoviral vector(BB-102) expressing the human wild type p53,GM-CSF and B7-1 genes into hepatocellular carcinoma(HCC) cell lines in vitro. Methods HCC cell lines were transferred with mock adenoviral vector expressing green fluorescent protein(Ad-GFP) at a series concentrations of MOI. p53, GM-CSF, and B7-1 expressing in HCC cells were determined by western blot, ELISA method and flow-cytometric analyses respectively. Results When 50...