Expression of Protein Kinase Calpha, CyclinD1 and Cdk4 Proteins in Colorectal Carcinomas and Their Clinical Signif icance

Objective 　To investigate the relationship between the expression of PKC2α、CyclinD1 and Cdk4 and the clinical significance in colorectal carcinomas. Methods 　Immunohistochemical staining was used to detect the expressions of PKC2α,CyclinD1 and Cdk4 in normal colorectal tissues, adenoma and carcinoma, respectively. Results 　The Positive rates of PKC2α expression in normal colorectal tissues, adenoma and carcinomas were 20 %, 47 %, 66. 0 %, respectively ( P < 0. 05), associated with the tumor cellular differentiation, Dukes phase and lymph node metastasis and CEA, respectively ( P < 0. 05) . Overexpression of CyclinD1 and Cdk4 were 55. 3 %,59. 6 % in colorectal carcinomas, showed an increasing tendency in colorectal carcinogenesis, respective2 ly ( P < 0. 05) ; the expression of CyclinD1 was correlated closely with differentiated degree, Dukes phage, lymph node metastasis and CEA, respectively ( P < 0. 05) ;the expression of Cdk4 was associated with differen2 tiated degree, Dukes phase and CEA, respectively ( P < 0. 05) . There is a positive relationship between PKC2α and CyclinD1 ( r = 0. 348, P < 0. 05), There is a positive relationship between CyclinD1 and Cdk4 ( r = 0. 393, P < 0. 05) . Conclusion 　Overexpression of PKC2α、CyclinD1 and Cdk4 may cont ribute to the pathogenesis of colorectal carcinoma, and it was correlated closely with differentiated degree, Dukes phase and CEA.