RNA Interference-mediated Significant Enhancement of Apoptosis in Yunnan Gejiu Lung Squamous Carcinoma Cells(YTMLC) by siRNA Expression Vector Targeting Survivin Gene

Objective To apply the small interfering RNAs targeting survivin to inhibit expression of endogenous survivin gene in Yunnan Gejiu lung squamous carcinoma cells(YTMLC) and increase the apoptosis of Yunnan Gejiu lung squamous carcinoma cells(YTMLC). Methods The recombinant plasmid pshRNA-survivin-1 and pshRNA-survivin-2 were constructed and transfected into YTMLC cell line.The expression level of survivin was determined by immunofluorescence staining and the transcription of survivin gene was detected by semi...