The Effect of HCV NS4B on Expressions of c-myc Protein and ras Protein in Hepatic Cells

Objective To investigate the effect of hepatitis C virus non-structural protein 4B(HCV NS4B)on c-myc and ras gene expressions in hepatic cells,and to study the possible role in the carcinogenesis of hepatoma. Methods The experiment was divided into negative control、empty vector PCXN2 and PCXN2-NS4B.The recombinant plasmid (PCXN2-NS4B) and the empty vector were transfected into Chang liver cells with liposome.Screening was performed with G418.HCV NS4B mRNA was detected by reversetranscription PCR.The protein ...