Effects of Peptide Nucleic Acids Targeting Ki67 on the Prol iferation and Apoptosis of Human Renal Carcinoma Cell Line

Objective 　To investigate the effects of peptide nucieic acid stargeting Ki67 gene ( PNAs) on the proliferation and apoptosis of human renal carcinoma cell line cells. Methods 　Human renal carcinoma cell line 786-0 cells were t reated with PNAs (10. 0μmol/ L) . The Ki67 expression of 786-0 cells was detected by immunohistochemical technique and Western blot method respectively. The proliferation of 786-0 cells was studied by cell growth curves and 3>> H-thymidinuptake assay. The apoptosis of 786-0 cells was detected by TUNEL assay. The cont ral group was t reated with antisense oligonucleotides (ASODNs) of Ki67. Results 　The Ki67 experssion rate of 786-0 cells t reated by PNAs (16. 9 ±0. 7) was lower than that of ASODNs t reated group (28. 6 ±0. 4) ( P < 0. 01) . The Ki67 protein rate of 78620 cells t reated by PNAs (42. 1 ±2. 2) was lower than that of ASODNs t reated group (83. 6 ±1. 4) ( P < 0. 01) . The 3 >>H-thymidine incorporation rates of 78620 cells t reated by PNAs (20. 7 ±1. 5) was lower than that of ASODNs t reated group (58. 6 ±1. 4) ( P < 0. 01) . The apoptosis rate of 786-0 cells t reated by PNAs (28. 7 ±2. 3) was higher than that of ASODNs treated group (13. 8 ±1. 0) ( P < 0. 01) . Conclusion 　PNAs have both antisense and antigene effcct s. PNAs of Ki67 have more powerful effect s on the proliferation and apoptosis of human renal carcinoma cells than ASODNs of Ki67 gene.