Synergistic Inhibitory Effect of survivin siRNA in Combination with 5-Fu on Inhibiting Proliferation of MCF-7 Cells

Objective To investigate the effect of the small interfering RNA(siRNA) targeted to survivin in combination with 5-Fu on inhibition the of MCF-7 cells proliferation. Methods A siRNA targeted to survivin was synthesized.siRNA was transfected into MCF-7 by lipofectin.Cell growth activity was evaluated by MTT assay.SAS software and Jin Zhenjun Method were used to evaluate the combination effects of siRNA and 5-Fu. Results Combination treatment with 5 nmol/L siRNA reduced the IC50 of 5-Fu from 4.42 μg/ml to 1.18 18μg/ ml ; the inhibitory of combination t reatment on MCF27 cells was higher than that of 5-Fu alone ( F = 26. 74, P < 0. 01. And synergism(Q ≥1. 15) was observed at the lower concent ration of 52Fu with combination of siRNA. Conclusion 　siRNA may enhance the effectiveness of 5-Fu on inhibiting the proliferation of MCF-7 cells.