Inhibitory Effects of Sulindac and Nimesulide on Cell Proliferation and COX-2, NF-κB Expression in Human Gastric Carcinoma Cell Line SGC-7901

Objective 　To investigate the inhibitory effects of sulindac and nimesulide on cell proliferation and COX-2, NF-κB expression in human gast ric carcinoma cells. Methods 　SGC-7901 cells were treated by sulindac and nimesulide. The anti-proliferative effect was measured by using MTT assay ;COX-2 and NF-κB protein expression were examined by immunohistochemical stain and western-blot analysis. Results 　Tumor cells proliferation was inhibited in dose and time dependent manner. The expression of COX-2, NF-κB in 100, 200μmol/ L nimesulide groups and 0. 6, 1. 2mmol/ L Sulindac groups were significantly decreased than the negative group ( P < 0. 05) . There was positive correlation between COX22 and NF2κB protein expression ( P < 0. 05) . Conclusion 　The proliferation of SGC-7901 cells was inhibited after treated by sulindac and nimesulide. Suppressing COX-2, NF-κB expression may play some roles in the antitumor process of these two drugs.