Relationship of Expression of HIF-1α and Glut-1 with Angiogenesis in Breast Carcinoma

Objective To study the relationship of the expression of HIF-1αand Glut-1 with Angiogenesis in breast carcinoma,and to analyze the correlation of them. Methods HIF-1α and Glut-1 protein expression were detected by immunohistochemical methods in 60 cases of breast carcinoma and 20 cases of tumor adjacent normal breast tissue.CD34 marking vascular endothelialcell was used to measure the microvascular density(MVD). Results The positive expression rates of HIF-1α and Glut1 in breast carcinoma was 68.3% and 63.3%,showing a significantly difference in the expression of HIF-1αand Glut-1 between breast carcinoma and cont rol group ( P < 0. 01), Expression of HIF-1αwas positively correlated with expression of Glut-1 ( r = 0. 461, P < 0. 001) . MVD in HIF-1α( + ) or Glut-1 ( + ) group was significantly higher than that in HIF-1α( - ) or Glut-1 ( - ) group ( P < 0. 01) . The MVD reached the maximum in patient s whose HIF-1αand Glut-1 were both positive ( P < 0. 01) . The expression of HIF-1α, Glut-1 and MVD were significantly correlated with lymphatic metastasis and surgical-pathologic staging ( P <0. 01) . Conclusion 　There have overexpresion of HIF-1αand Glut-1 in breast carcinoma, they may have a synergistic effect in the genesis and development of breast carcinoma, and may play an important role in angiogenesis of breast carcinoma.