HIF-1α Gene Antisense Oligodexynucleotide Enhances the Sensitivity of Gastric Carcinoma Cells to Cisplatin

Objective To investigate the effects of antisense oligodeoxynucleotide targeting HIF-1α on apoptosis and chemotherapy sensitivity of gastric carcinoma SGC-7901. Methods Antisense ODN and sense ODN were constructed and transfected into SGC-7901 cells by Dosper liposomal reagent.The changes of HIF-1α gene expression were observed by RT-PCR and immunocytochemistry.The cytotoxicity of Cisplatin was determined by MTT,while apoptosis indexes were detected by using terminal deoxynucleotidyl transferase-mediated nic...