Cloning and Identification of Caspase-3 Gene from HepG2 Cells that Underwent Autophagic Apoptosis

Objective To acquire the fragment of caspase-3 gene from HepG2 cells, a human hepatocarcinoma cell line,that underwent autophagic apoptosis induced by vincristine(VCR). Methods Total RNA was extracted from the HepG2 cell line,then the fragment of caspase-3 gene from the cells was amplified by RT-PCR and it was cloned,sequenced by gene recombination techniques for confirmation. Results The prospective sequence of selected cloned caspase-3 gene was 625 bp.It is consistent with that of reported human caspase-3 g...