Experimental Study on the Suppression of the Invasion and Metastasis of Osteosarcoma by UTI

Objective In order to investigate the effect of invasion and metastasis of human osteosarcoma MG-63 by the urinary trypsin inhibitor(UTI) in vitro. Methods Different doses(10、50、100、300、500nM) of UTI were added into the human osteosarcoma MG-63 medium and 50μl PBS was added into the control at the same time,then the expression of urokinase type plasminogen activator(UPA) was detected by immunohisto-chemistry,RT-PCR and Western blot. Results The more concentration of UTI was transfected,the lesser UPA were exp...