Large-scale Generation of Mature Clinical Grade Dendritic Cells

Objective To study the method for generation large scale mature dendritic cells from nonproliferation progenitors in patient s blood. Methods The procedure uses 5% AB human plasma in the place of 10% fetal calf serum and involves two steps. The first step is to work on a 5～7 day culture of plastic adherent blood monocyte cells in medium supplemented rhGM CSF and rhIL 4. The second step is to expose to monocyte conditioned medium mimic or TNF α. Results There was no significant difference observed in...