Experimental Study of Vaccination with Dendritic Cells Pulsed with Tumor Cell Lysates for Immunotherapy of C_6 Glioma

Objective To investigate effect of dendritic cells pulsed with tumor antigens which are used to treat with intracranial glioma. Methods dendritic cells were isolated from rat bone marrow precursors stimulated in vitro with granulocyte macrophage colony stimulating factor (GM CSF) and interleukin 4 (IL 4).Then mature DCs were analyzed; Produce lysates and dendritic cells vaccinum; then we established animal model treated with dendritic cells vaccinum. The rat brains were then removed and examined hist...