Effects of Liposome-c-raf-1 Antisense Oligodeoxynucleotides Transfection on the HumanOvarian Cancer Transplanted Subcutaneously in Nude Mice

Objective 　To investigate the effect s of liposome-c-raf-1 antisense phosphoro-thioate oligodeoxy-nucleotides (c-raf-1 ASODN) on the human ovarian epithelial cancer t ransplanted subcutaneously in nude mice. Methods 　Human ovarian epithelial cancer cell lines SKOV3 were t reated with liposome-c-raf-1 ASODN, then transplanted subcutaneously in nude mice. The Changes of tumor volume were observed and the tumor growth inhibitory rate was calculated. Results 　The tumorigenic ability of the t reated cell lines was reduced, the maximum tumor growth inhibitory rate was 35. 3 %( P < 0. 05) . The first time that tumor can be detected was increased up to 8. 8 days ( P < 0. 05) . Conclusion 　The results suggested that c-raf-1 ASODN transfection can inhibit the tumorigenesis of ovarian epithelial cancer cell lines in nude mice, it may be a useful gene therapy for the ovarian epithelial carcinoma.