Inhibition of Transfected PTEN on Human Colon Cancer

Objective 　To study the inhibitory effect of transfected PTEN on LoVo cells. Methods 　The human PTEN cDNA was t ransferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analysized by Western blot and flow cytometry. Before or af ter t ransfection, the activities of 5-Fu in inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT. Results 　PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in t ransfected LoVo cell. Conclusion 　Transfected PTEN remarkably up-regulated PTEN expression in LoVo cells and promoted the apoptosis. PTEN transfection associated with 5-Fu t reatment had cooperatively cytotoxic effect .