Inhibitory Effect of Quercetin in Combination with Tetramethylpyrazine on Growth of Lung Adenocarcinoma in Mice

Objective 　To explore the inhibitor effect and its mechanism of quercetin combination with tetramethylpyrazine on growth of lung carcinoma in mice. Methods 　The Lewis lung carcinoma models of C₅₇ BL mice were establish. Forty C₅₇BL mice were randomly divided into 4 groups : control group ( Group A), quercetin group (Group B), tetramethylpyrazine group (Group C) and quercetin + tetramethylpyrazine group (Group D) .Different treatments were served from day 2 after transplantation and all mice were sacrificed after 22 days. The volumes of tumors were measure respectively during the therapy time ; subcutaneous tumors were processed for histological examination. The expression of microvessel density (MVD), proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) were detected by immunhistochemical method. The apoptosis index (AI) was measured by biotinyated-dUTP nick and labeling ( TUNEL) . Results 　(1) The tumor growth was suppressed significantly in Group B, C and D. The weights of tumor were markedly decreased in Group B, C and D compared with Group A. Group B, C, D had a growth inhibition rate of 39. 87 %,35. 45 % and 54. 58 % respectively. (2) The expression levels of VEGF, MVD and PCNA were significantly lower in Group B, C, D than group A. AI was enhanced in Group B, C, D, compared with Group A. Conclusion 　Quercetin combination with tetramethylpyrazine can remarkably inhibit the growth of Lewis lung carcinoma in mice, and its mechanisms might be relative to inhibiting the angiogenesis and tumor proliferating and inducing apoptosis.