Association of BH3 only Proteins with Etoposide-Induced Apoptosis of Gastric Adenocarcinoma Cells

Objective To study the potential role of BH3-only proteins of the bcl-2 family in Etoposide-induced apoptosis of gastric adenocarcinoma cells. Methods FITC-conjugated Annexin V staining was used to detected apoptotic cells in flow cytometry.The expression levels of mRNA for BH3-only proteins were measured by semi-quantitative RT-PCR. Results Etoposide induced apoptosis of gastric adenocarcinoma cells in a dose-and time-dependent manner.It appeared that SGC-7901 cells are more sensitive to the compound than BG...Objective To study the potential role of BH3-only proteins of the bcl-2 family in Etoposide-induced apoptosis of gastric adenocarcinoma cells. Methods FITC-conjugated Annexin V staining was used to detected apoptotic cells in flow cytometry.The expression levels of mRNA for BH3-only proteins were measured by semi-quantitative RT-PCR. Results Etoposide induced apoptosis of gastric adenocarcinoma cells in a dose-and time-dependent manner.It appeared that SGC-7901 cells are more sensitive to the compound than BG...