Mutation Analysis of Transforming Growth Factor β Ⅱ Receptor Gene in Colorectal Adenoma Carcinogenesis
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Abstract
Objective To determine the relationship between the mutation of the TGF-β type Ⅱ receptor gene and microsatellite instability(MSI)in the tumorigenesis of colorectal cancer. Methods Selecting information-integrated fresh specimens of 60 colorectal carcinomas and 42 adenomas,the genomic DNA was extracted by phenol-chloroform procedure.Selecting BAT-26 as microsatellite marker to determine MSI of colorectal cancer,The mutation hot spot cDNA709-718 of TGF-β R Ⅱ was detected using silver-staining PCR-SSCP(single ...
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