Inhibitoy Effect of Arsenic Trioxide on Gastric Cancer Cell SGC-7901 During Hypoxia

Objective 　To investigate the effect of arsenic t rioxide (As₂O₃ ) on gast ric cancer cell SGC-7901 during the normal and hypoxia. Methods 　The environment of hypoxia was established by GasPak method. The effect of As₂O₃ was determined by MTT, and FCM was used for studying the apoptosis of SGC-7901 and cell cycle. Results 　As₂O₃ can inhibit significantly the growth of SGC-7901, and arrest the cell in G2/M and S phases. But this inhibitory effect was decreased significantly during hypoxia ( P < 0. 01), which was found by the lower percentage of apoptosis. Conclusion 　As₂O₃ can inhibit the growth of gastric cancer cell, but during hypoxia this inhibitory effect was decreased, so it may be limited to cure hypostatic neoplasm for only using As₂O₃ .