The Research of Endostatin Gene Therapy on Mouse Hepatoma and the Mechanism
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Abstract
Objective To study the inhibitory effect of endostatin gene therapy on mouse hepatoma and the mechanism of which. Methods BHK 21 was transfected with pSecES, in the supernant of which endostatin was assayed by ELISA and the supernant was used to culture endothelium cells ECV304. Proliferation of the latter was evaluated by MTT. Then H22 cells were inoculated into the leg muscle of mouse, which was injected with pSecES/PVP repeatedly. Tumor weight and serum endostatin were assayed. Tumor microvessel density a...
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