Specific Anti-tumor Immunological Effect by Dendritic Cell Transfected with GM-ΔSur Gene

Objective To study the specific anti-tumor immunological effects induced by dendritic cells transfected with GM-CSF gene and survivin gene(GM-ΔSur) in vitro. Methods pcDNA 3.1 plasmid containing GM-ΔSur fusion gene was constructed and was transfected into dendritic cells by jetPEITM-Mcrophage transfected kit; cellular surface phenotype such as CD1a, CD80, CD86, CD83 were detected by FACS; The specific cytotoxicity of induced human cytotoxic T lymphocyte (CTLs) to tumor cells was detected with LDH assay. Res...