Folic Acid-Modified Liposome Quercetin Induces Apoptosis of Triple-Negative Breast Cancer Cells via Mitochondrial Apoptosis Mediated by JAK2/STAT3 Signaling Pathway

CHEN Fengxia; PU Feifei

doi:10.3971/j.issn.1000-8578.2024.23.1390

Cancer Research on Prevention and Treatment > 2024 > 51(7): 554-560. > DOI: 10.3971/j.issn.1000-8578.2024.23.1390

CHEN Fengxia, PU Feifei. Folic Acid-Modified Liposome Quercetin Induces Apoptosis of Triple-Negative Breast Cancer Cells via Mitochondrial Apoptosis Mediated by JAK2/STAT3 Signaling Pathway[J]. Cancer Research on Prevention and Treatment, 2024, 51(7): 554-560. DOI: 10.3971/j.issn.1000-8578.2024.23.1390

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Folic Acid-Modified Liposome Quercetin Induces Apoptosis of Triple-Negative Breast Cancer Cells via Mitochondrial Apoptosis Mediated by JAK2/STAT3 Signaling Pathway

CHEN Fengxia^{1, 2, 3,},
PU Feifei^4, , ,

1.
Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
2.
Hubei Provincial Clinical Research Center for Cancer, Wuhan 430071, China
3.
Hubei Key Laboratory of Tumor Biological Behavior, Wuhan 430071, China
4.
Department of Orthopedics, Wuhan Hospital of Traditional Chinese and Western Medicine (Wuhan No.1 Hospital), Wuhan 430022, China

Funding: National Natural Science Foundation of China (No. 82274559); Fundamental Research Funds for the Central Universities (No. 2042023kf0052)

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Corresponding author:
PU Feifei, E-mail: pufeifei@hust.edu.cn
Received Date: January 03, 2024
Revised Date: April 28, 2024
Available Online: August 11, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of folic acid–modified liposome quercetin (FLQ) on the proliferation and apoptosis of triple negative breast cancer (TNBC) cells and explore its underlying mechanism.
Methods
CCK-8 was used to detect the effect of FLQ on TNBC cell viability. Colony formation assay was conducted to detect the effect of FLQ on TNBC cell proliferation. Flow cytometry was performed to detect the effect of FLQ on TNBC cell apoptosis, the levels of intracellular ROS, and mitochondrial membrane potential. Western blot analysis was conducted to detect the expression levels of JAK2/STAT3 signaling pathway-related and apoptosis-related proteins.
Results
FLQ inhibited the proliferation and promoted the apoptosis of MDA-MB-231 cells (P=0.023, P<0.001). It promoted mitochondrial membrane potential collapse and increased the intracellular ROS levels of MDA-MB-231 cells (P=0.003, P=0.034); inhibited the phosphorylation levels of JAK2 and STAT3; upregulated the expression levels of the proapoptotic proteins Bax, Bak, cytochrome C, and Cleaved-Caspase-3 (P<0.001, P<0.001); and downregulated the expression levels of the antiapoptotic proteins Bcl2 and Bcl-x_L (P=0.037, 0.028).
Conclusion
FLQ inhibits the proliferation and induces the apoptosis of MDA-MB-231 cells. These effects may be related to the activation of the mitochondrial apoptosis pathway through the inhibition of the JAK2/STAT3 signaling pathway.

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Competing interests: The authors declare that they have no competing interests.

References (27)

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Folic Acid-Modified Liposome Quercetin Induces Apoptosis of Triple-Negative Breast Cancer Cells via Mitochondrial Apoptosis Mediated by JAK2/STAT3 Signaling Pathway