AAMP Promotes Osteosarcoma Cell Metastasis by Regulating YAP Signaling Pathway

DENG Qianrong; ZHAN Fangbiao; XIE Chaozheng; XIANG Shuang; CHEN Jian; YANG Yi

doi:10.3971/j.issn.1000-8578.2024.23.1034

Cancer Research on Prevention and Treatment > 2024 > 51(6): 440-447. > DOI: 10.3971/j.issn.1000-8578.2024.23.1034

DENG Qianrong, ZHAN Fangbiao, XIE Chaozheng, XIANG Shuang, CHEN Jian, YANG Yi. AAMP Promotes Osteosarcoma Cell Metastasis by Regulating YAP Signaling Pathway[J]. Cancer Research on Prevention and Treatment, 2024, 51(6): 440-447. DOI: 10.3971/j.issn.1000-8578.2024.23.1034

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AAMP Promotes Osteosarcoma Cell Metastasis by Regulating YAP Signaling Pathway

DENG Qianrong^{1, 2,},
ZHAN Fangbiao^{1, 3, *,},
XIE Chaozheng⁴,
XIANG Shuang^{1, 5},
CHEN Jian^{1, 3},
YANG Yi^{1, 6, , ,}

1.
Postgraduate Training Base of Jinzhou Medical University, Chongqing University Three Gorges Hospital, Chongqing 404000, China
2.
Health Management Center, Chongqing University Three Gorges Hospital, Chongqing 404000, China
3.
Department of Orthopedics, Chongqing University Three Gorges Hospital, Chongqing 404000, China
4.
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
5.
Department of Pathology, Chongqing University Three Gorges Hospital, Chongqing 404000, China
6.
Department of Hematology, Chongqing University Three Gorges Hospital, Chongqing 404000, China

Funding: The General Project of Chongqing Natural Science Foundation (No. cstc2021jcyj msxmX0909); The Young and Middle-aged Medical High-end Talent Project of Chongqing (No. 33158); The Key Project for Young Talents of Chongqing University Three Gorges Hospital (No. 2022YJKYXM-043); The Wanzhou District of Chongqing PhD Zhitongche Research Project (No. wzstc20230404)

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Corresponding author:
YANG Yi, E-mail: 112182701@qq.com
Received Date: September 24, 2023
Revised Date: December 04, 2023
Available Online: June 19, 2024

Graphical Abstract

Abstract

Abstract

Objective
To determine the role of AAMP in osteosarcoma cells and explore the mechanism of invasion and metastasis of osteosarcoma cells regulated by AAMP through the YAP signaling pathway.
Methods
Public sequencing data analysis was used to explore the correlation between AAMP and osteosarcoma. q-PCR, Western blot, and immunohistochemistry were used to detect the expression levels of osteosarcoma cell-related molecules. CCK-8 was used to detect cell proliferation ability. Transwell and wound healing assays were used to detect the invasive and metastatic abilities of osteosarcoma cells. Immunofluorescence was used to detect the cell localization and expression levels of related molecules.
Results
High expression of AAMP was negatively correlated with the prognosis of patients with osteosarcoma (P<0.05), and the expression of AAMP in patients with metastatic osteosarcoma increased (P<0.05). Compared with the control group, the AAMP interference group showed significantly decreased migratory, invasive, and EMT activities (P<0.05). The expression of p-CFL1 reduced after the knockdown of AAMP, and the cell plate pseudopods decreased significantly (P<0.05). A positive correlation was found between the expression levels of AAMP and YAP in osteosarcoma cells (P<0.05). Interfering with YAP expression can affect the migration and invasion of osteosarcoma cells.
Conclusion
AAMP promotes osteosarcoma cell metastasis by regulating the YAP signaling pathway, suggesting that AAMP may be a key molecule in promoting invasion and metastasis of osteosarcoma.
- Osteosarcoma,
- AAMP,
- YAP,
- CFL1,
- Migration,
- Invasion,
- Pseudopodium

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Competing interests: The authors declare that they have no competing interests.

References (25)

References

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AAMP Promotes Osteosarcoma Cell Metastasis by Regulating YAP Signaling Pathway