可调控重组腺相关病毒对MCF-7细胞基因转染效率及表达的研究

Zeng Zhao-jun; LI Zi-bo; HU Wei-xin; LUO Sai-qun; Chen Qian

doi:10.3971/j.issn.1000-8578.2024

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Cancer Research on Prevention and Treatment > 2007 > 34(09): 667-670. > DOI: 10.3971/j.issn.1000-8578.2024

Zeng Zhao-jun, LI Zi-bo, HU Wei-xin, LUO Sai-qun, Chen Qian. 可调控重组腺相关病毒对MCF-7细胞基因转染效率及表达的研究[J]. Cancer Research on Prevention and Treatment, 2007, 34(09): 667-670. DOI: 10.3971/j.issn.1000-8578.2024

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可调控重组腺相关病毒对MCF-7细胞基因转染效率及表达的研究

Molecular Biology Research Center, College of Biological Science and Technology, Central South University, Changsha 410078, China

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Corresponding author:
HU Wei-xin
Received Date: May 24, 2006
Revised Date: September 03, 2006

Abstract

Abstract

Objective To explore the treatment of human breast cancer by adeno-associated virus (AAV) expressing HSVtk gene under the regulation of Tet regulatory system, the recombinant AAV-2 were transduced into MCF-7 cell line. Methods The Tet-On and HSVtk gene was respectively inserted into the AAV cassettes(pAAV MCS),then rAAV/HSVtk/Tet-On were generated by co-transfection 293 cells with other plasmids(pAAV-RC、pHelper). The viral titer of rAAV/HSVtk/Tet-On and its expression were measured by β-galactosidase staini...
- Adeno-associated virus,
- Tet-On gene,
- HSVtk gene,
- Breast neoplasms,
- Gene therapy

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Chen Qian

Molecular Biology Research Center, College of Biological Science and Technology, Central South University, Changsha 410078, China

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可调控重组腺相关病毒对MCF-7细胞基因转染效率及表达的研究

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可调控重组腺相关病毒对MCF-7细胞基因转染效率及表达的研究

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